RFP-HBMMSCs are selected from puromycin resistant HBMMSCs after transfected with RFP expressing lentiviral particles. RFP-HBMMSCs are supplied in cryovials at passage 3- 4. Each lot of RFP-HBMMSCs is tested to ensure the cells can be passaged at least for three times (i.e., approximately 9 to 10 population doublings) after thaw. RFP-HBMMSCs should be maintained in complete growth media (Mesenchymal Stem Cell Growth Medium (MSCGM)) following the detailed protocol described below.RFP慢病毒颗粒转染后,从嘌呤霉素耐药HBMMSCs中选择RFP-HBMMSCs。RFP-HBMMSCs在3- 4通道的低温容器中供应。对每个批次的RFP-HBMMSCs进行测试,以确保细胞在解冻后至少能传代三次(即约9 - 10倍数量)。RFP-HBMMSCs应按照以下详细方案在完全生长培养基(间充质干细胞生长培养基(MSCGM))中维持。