Lane 1, 100bp DNA ladder lane 2, M. pneumoniae DNA 10 pg lane 3, M. pneumoniae DNA 1 pg lane 4, M. pneumoniae DNA 100 fg lane 5, M. pneumoniae DNA 10 fg lane 6, M. pneumoniae DNA 1 fg lane 7, Positive control lane 8, 9, Negative control lane 10, M. pneumoniae DNA 10 pg lane 11, M. pneumoniae DNA 1 pg lane 12, M. pneumoniae DNA 100 fg lane 13, M. pneumoniae DNA 10 fg lane 14, M. pneumoniae DNA 1 fg lane 15, Positive control lane 16, 17, Negative control