Mycoplasma contamination screening in R&D cell lines
Contains the main contaminants of the cell culture including M. orale, M. hyorhinis, M. arginini, M. fermentans, M. hominis, Acholeplasma laidlawii, allowing all mycoplasma detection of M. pneumoniae, M. salivarium, M. genitalium, M. penentrans and ureaplasma
The primer binds to the highly conserved 16s rRNA coding region
DNA of eukaryotes and other bacterial species are not detected
PCR performance monitoring is possible with the application of internal amplification control (IAC)
Optimized PCR Premix provided for easy-to-use
Conventional PCR technique applied
Template DNA preparation through boiling of the supernatant of the cell culture
Sensitivity: 10–100 fg/reaction
The test results are within 2~3 hours
Lane 1, 100bp DNA ladder lane 2, M. pneumoniae DNA 10 pg lane 3, M. pneumoniae DNA 1 pg lane 4, M. pneumoniae DNA 100 fg lane 5, M. pneumoniae DNA 10 fg lane 6, M. pneumoniae DNA 1 fg lane 7, Positive control lane 8, 9, Negative control lane 10, M. pneumoniae DNA 10 pg lane 11, M. pneumoniae DNA 1 pg lane 12, M. pneumoniae DNA 100 fg lane 13, M. pneumoniae DNA 10 fg lane 14, M. pneumoniae DNA 1 fg lane 15, Positive control lane 16, 17, Negative control