人脑微血管内皮细胞(HBMEC)
Primary Human Brain Microvascular Endothelial Cells,These antibody-free human primary cells were initiated by elutriation of dispase-dissociated normal human brain cortex tissue without the use of positive selection of antibodies.人脑微血管内皮细胞取材于正常人脑皮层组织,dispase-dissociated技术提取纯化,细胞无抗性。
动物种别/Organism:人
组织来源 Tissue and Cell Type:大脑皮层组织
形态/Morphology:小石子路状,贴壁生长
培养基和添加剂/Complete Growth Medium and Culture Conditions:
完全培养基:CultureBoost™经典细胞培养基(货号:4N0-500)
气相:空气,95%;二氧化碳,5%。 温度:37摄氏度。
冻存液:细胞冻存液Cell Freezing Medium(4Z0-705)
人脑微血管内皮细胞(HBMEC)出库检测指标:
Standard Tests |
TESTS |
RESULTS |
HIV Serologic Test (donor level HIV AB EIA) |
Negative |
HIV PCR Test (frozen cell pool by CLIA Licensed Clinical Lab) |
Negative |
Hepatitis B (HBV) and Hepatitis C (HCV) PCR Test (at frozen cell level) |
Negative |
Test of frozen cells for Mycoplasma spp. (ATCC method by CLIA Licensed Clinical Lab) |
Negative |
Cell Markers and Functional Tests |
MARKER |
RESULTS |
CD31 |
> 98% positive by immunofluorescence |
VWF / Factor VIII |
> 95% positive by immunofluorescence |
Uptake of Di-I-Ac-LDL |
> 95% positive by immunofluorescence |
注意事项:此产品仅供科研使用
cell Systems由著名的细胞生物学家Carl Soderland于1989年在西雅图创立,是分离和制备原代细胞培养物的初创人之一。他们分离出来的原代细胞培养物被制造成“黄金”标准,在大量科学期刊上被引用了数十年。他们的原代细胞使用无抗体分选和培养技术,让实验的结果更为准确,更具有重复性。
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