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应用 cell culture | mammalian: suitable
颜色 yellow-white
溶解性 H2O: soluble 50 mg/mL, clear, colorless to faintly yellow
Puromycin is a broad spectrum antibiotic produced by Streptomyces alboniger. It is an analog of the 3′ end of aminoacyl-tRNA. It is an inhibitor of peptidyl transferase and is active in both prokaryotes and eukaryotes.
Chemical structure: peptidyl nucleoside
应用
Puromycin dihydrochloride is a part of the amino-nucleoside family of antibiotics and is derived from Streptomyces alboniger. It is a broad spectrum antibiotic with antitumor activity, as an inhibitor of protein synthesis and has been used to study transcription regulatory mechanisms that control the sequential and coordinate expression of genes during cell differentiation. The recommended working concentration in eukaryotic cell culture is 1-10 μg/mL.
Puromycin dihydrochloride from Streptomyces alboniger has been used:
in the preparation of puromycin stock solution for puromycin sensitivity assay.
to select lentivirus-infected cells containing puromycin resistant selection marker.
Used as a selective agent for cells that contain the resistance gene puromycin N-acetyl-transferase (PAC). Recommended for use at a range of 1-10 μg/ml. Sterilize stock solution by filtration using 0.22 μm filter then store in aliquots at −20 °C.
包装
10, 25, 100 mg in glass bottle
生化/生理作用
Puromycin associates in a non-specific manner with growing polypeptide chains, and thus, leads to premature termination of translation. It inhibits protein synthesis in two ways. It associates with the donor substrate, peptidyl-tRNA, in the P site and thus, functions as an acceptor substrate. Second, it competes with aminoacyl tRNA to bind with the A′ site within the peptidyl transferase center.
作用机制:嘌呤霉素通过提前终止链来抑制蛋白质合成,作为氨基酰基-tRNA 的 3′ 末端的类似物起作用。
As supplied, this product remains active for four years when stored at -20°C.
制备说明
This product is soluble in water at 50 mg/mL. A stock solution should be prepared by filtration using a 0.22 μm filter and then stored in aliquots at -20°C. This product is also soluble in methanol at 10 mg/mL.
其他说明
Keep container tightly closed in a dry and well-ventilated place.Keep in a dry place