Gentle Cell Dissociation Reagent (GCDR) is an enzyme-free reagent suitable for the dissociation of human embryonic stem (ES) cells or human induced pluripotent stem (iPS) cells into cell aggregates for routine passaging or into a single-cell suspension.
GCDR is also suitable for the isolation of intestinal crypts to establish intestinal organoids, and for breaking up Corning® Matrigel® domes when passaging organoid cultures. GCDR does not contain enzymes or other proteins.
GCDR is available individually or in a kit (Catalog #07190) with Vitronectin XF™, CellAdhere™ Dilution Buffer, and Non Tissue Culture-Treated 6-Well Plates.
Advantages: • Enzyme-free and chemically defined
• Gentle on cells
• High expansion of human ES/iPS cells during routine culture
• Simple, room temperature passaging protocols