PancreaCult™ Organoid Growth Medium (Mouse) is a serum-free, defined cell culture medium for establishment and maintenance of mouse pancreatic exocrine organoids. These organoids, or "mini-pancreata", provide an in vitro organotypic culture system for studying pancreatic cell biology, disease, and cancer. Organoids grown in PancreaCult™ feature an epithelium expressing genes marking pancreatic stem cells (LGR5), progenitor cells (PDX1, SOX9), and ductal cells (CAR2, MUC1, KRT19). Pancreatic organoids can be passaged every 3 - 6 days for long-term maintenance and can also be cryopreserved.
PancreaCult™ supports mouse pancreatic organoid culture either embedded in Corning® Matrigel® domes or in a dilute Matrigel® suspension. Organoid culture enables convenient in vitro characterization of the pancreatic epithelium in a physiologically relevant system and reduces the need for animal use.
Advantages: • Convenient in vitro system for generating organoids within one week • Detailed, step-by-step protocol with no injury models, hand-picking of ducts, or cell sorting required • Simple, two-component format; serum-free and defined medium formulation • Flexible protocol for generation and maintenance of organoid cultures in matrix domes or suspension culture
Components: • PancreaCult™ OGM Mouse Basal Medium, 95 mL • PancreaCult™ OGM Mouse Supplement, 5 mL
