The EasySep™ Direct HLA Crossmatch B Cell Isolation Kit can be used to isolate highly purified B cells directly from human whole blood, spleen, or lymph nodes by immunomagnetic negative selection. This cell isolation kit targets non-B cells for removal with antibodies recognizing specific surface markers. Unwanted cells are labeled with antibodies and EasySep™ Direct RapidSpheres™, and separated using an EasySep™ magnet. Desired cells are simply collected into a new tube and and are immediately available for downstream applications such as flow cytometry crossmatch.
Advantages:
• > 99.9% RBC depletion without the need for density gradient centrifugation, sedimentation, or lysis
• Up to 99.8% purity of isolated cells
• Fast, easy-to-use, and column-free
• Isolated cells are untouched
Components:
EasySep™ Direct HLA Crossmatch B Cell Isolation Kit (Catalog #19684)
EasySep™ Direct HLA Crossmatch B Cell Isolation Cocktail, 2 x 2.5 mL