Western blotting: 1-5µg/mL Immunocytochemistry in formalin fixed cells: 5-20µg/mL Immunohistochemistry in formalin fixed frozen section: 5-20µg/mL Immunohistochemistry in paraffin section: 5-20µg/mL Enzyme-linked Immunosorbent Assay: 0.05-2µg/mL Optimal working dilutions must be determined by end user.