参考文献:de Smit MH, van Duin J. Secondary structure of the ribosome binding site determines translational efficiency: a quantitative analysis. Proc Natl Acad Sci U S A. 1990; 87:7668–7672.
剪切位点的去除 去除内含子属性的剪接位点,提高蛋白表达成功率。 参考文献:Pagani F, Raponi M, Baralle FE. Synonymous mutations in CFTR exon 12 affect splicing and are not neutral in evolution. Proc Natl Acad Sci U S A. 2005; 102:6368–6372.