Chemokine(C motif)ligand(XCL1),as known as lymphotactin,is the only known member of the C-chemokine family and signals through the receptor XCR1,formally known as GPR5.The expression of lymphotactin is abundant in some activated T cells such as activated CD8+ T cells and other class I MHC restricted T cells.It is found in high levels in spleen,thymus,intestine and peripheral blood leukocytes,and at lower levels in lung,prostate gland and ovary.XCL1 induces its chemotactic function by binding to a chemokine receptor called XCR1.Recombinant Human XCL1 which is a single non-glycosylated polypeptide chains containing 92 amino acids and it shares approximately 60%amino acid sequence homology with the murine and rat protein.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
外观
Sterile Filtered White lyophilized(freeze-dried)powder.
纯度
>98%by SDS-PAGE and HPLC analyses.
来源
Escherichia coli.
生物活性
Fully biologically active when compared to standard.The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration of 10-100ng/ml.
成分
Lyophilized from a 0.2μmfiltered concentrated solution in 20mMPB,pH 7.4,150 mM NaCl.
内毒素
Less than 1 EU/ug of rHuLymphotactin/XCL1 as determined by LAL method.
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20?C.Further dilutions should be made in appropriate buffered solutions.
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