RS-1 is a RAD51-stimulatory compound, which increases the DNA binding activity of RAD51. It is an HDR(homology-directed repair) enhancer.
信号通路
Others
靶点
RAD51
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IC50
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体外研究
RS-1 stimulates the binding of hRAD51 to ssDNA. Low-micromolar concentrations of this small molecule enhance DNA binding and result in longer protein–DNA complex lengths. In addition, RS-1 stabilizes the active form of hRAD51 filaments and this is reflected in an enhanced strand assimilation activity.
体内研究
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临床实验
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特征
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相关实验数据(此数据来自于公开文献,信裕生物并不保证其有效性):
酶活性检测实验
方法
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细胞实验
细胞系
Early-passage neonatal human dermal fibroblasts
浓度
1-7.5μM
处理时间
24h
方法
Early-passage neonatal human dermal fibroblasts were maintained in Medium 106 supplemented with low serum growth supplement. Cell-survival experiments were performed using a sulforhodamine B assay. Cells are incubated for 24h in cisplatin with or without RS-1 and are subsequently incubated for an additional 6 days in normal media lacking both agents. Sulforhodamine B-stained cells are quantified with a Synergy plate reader.
动物实验
动物模型
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配制
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剂量
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给药方式
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参考文献: 1. Jayathilaka K, et al. Proc Natl Acad Sci USA. 2008, 105(41), 15848-53.
2. Song J, et al. Nat Commun. 2016, 7, 10548.
包装清单: