ScienCell’s qPCR Loading Indicator Kit (LIK) contains 3 inert loading indicators (LIs), LI Gold, LI Red and LI Blue, to be used in SYBR® Green or other green fluorescent dye-based qPCR reactions. It allows for better visualization and tracking of sample loading in qPCR plates or tubes.
LIK is validated with SYBR® Green dye-based qPCR, however, one or more components of this kit may work for other types of qPCR. Always do a parallel test with and without the loading indicators for feasibility before use.
Quality Control
LIK is validated with FastStart Essential DNA Green Master (Roche, Cat #06402712001), and at the suggested concentrations, no effect on qPCR specificity or efficiency was observed.