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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
- 库存:
100
- 宿主:
Rabbit
- 靶点:
peptide corresponding to amino acids 347 to 360 of human DNase II precursor (2-4)
- 抗体名:
DNASE2A, DNL2
- 规格:
50 μg
40kDa
细胞定位:
Antigen Cellular Localization: Lysosome.
形式:
Liquid
参考文献:
Yasuda T.,et al.Ann. Hum. Genet. 62:299-305(1998). Shiokawa D.,et al.Biochem. Biophys. Res. Commun. 247:864-869(1998). Baker K.P.,et al.Gene 215:281-289(1998). Krieser R.J.,et al.J. Biol. Chem. 273:30909-30914(1998). Yasuda T.,et al.J. Biol. Chem. 273:2610-2616(1998).
产品概述:
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality DNASE2 / DNase II antibody (aa347-360).
功能:
Hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. Plays a major role in the degradation of nuclear DNA in cellular apoptosis during development. Necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells.
总结:
Hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. Plays a major role in the degradation of nuclear DNA in cellular apoptosis during development. Necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells.
联系方式:
生物商城:http://www.annoron.com
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官方售后:400-965-8633
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文献和实验,000 x G for 1 minute 2) For each HiBind® RNA Minicolumn, prepare the DNase I digestion reaction mix as follows: OBI DNase I Digestion Buffer 73.5ul RNase-free DNase I (20 Kunitz unites/ul) 1.5ul Total volume 75ul
Protein A Purification of Antibody
1. Reagents(1) Affi-gel Protein-A Agarose (BioRad #153-6153)(2) MAPS II Binding Buffer (BioRad # 153-6161)(3) 0.314 g/ml diH2O(4) MAPS II Elution Buffer (BioRad #153-6162)(5) 0.023 g/ml diH2O(6) MAPS II Regeneration Buffer (BioRad #153-6166(7) 1M
Protein A Purification of Antibody
Reagents Affi-gel Protein-A Agarose (BioRad #153-6153) MAPS II Binding Buffer (BioRad # 153-6161) 0.314 g/ml diH2 O MAPS II Elution Buffer (BioRad #153-6162)
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