Human IL4/IL-4/Interleukin-4 ELISA Kit(产品说明)
反应种属:人
试剂盒靶点:IL4
试剂盒应用:ELISA
试剂盒保存:Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
试剂盒介绍:For the quantitative determination of Human IL4/IL-4/Interleukin-4 concentration in cell culture supernates.
试剂盒线性范围:10.94-700
试剂盒包被抗体类型:mouse mAb
试剂盒检测抗体类型:mouse mAbKIT11846
试剂盒检测灵敏度:2.54
试剂盒天然样本检测量:Human peripheral blood lymphocytes (PBLs) were isolated fromwhole blood using ficol-hypaque and passed through an R&D Systems T-Cell enrichmentcolumn. Eluted T-Cells were cultured at 2 x 106/mL in DMEM with 10% fetal calf serum,5 g/mL PHA, and 10 ng/mL rhIL-2. After 6 days of culture, cells were changed into DMEM with 10% fetal calf serum, 10 ng/mL PMA, 1 g/mL Ca2+ ionophore, and 5 g/mL PHA.Supernates were collected after 20 hours of stimulation and measured 70 pg/mL
试剂盒特异性:This assay recognizes both recombinant and natural Human IL4. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
试剂盒检测原理:Solid Phase Sandwich ELISA
试剂盒组成:1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
试剂盒检测样品:细胞上清液
1, Che, D; et al. p-Phenylenediamine induces immediate contact allergy and non-histaminergic itch via MRGPRX2. Chemico-biological interactions, Pubmed: 34826398