Description Superoxide dismutase (SOD), as one of the body?s most important defense mechanisms against freeradical damage, catalyzes the dismutation of the
superoxide radical (O2-) into hydrogen peroxide (H2O2) and elemental oxygen (O2). In ScienCell?s SOD Assay, the superoxide anions, generated from the conversion of xanthine to uric acid and hydrogen
peroxide by xanthine oxidase (XOD), reduce WST-1 (4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate sodium salt) to water-soluble formazans, which can be measured by absorbance at 438 nm. SODs lower the rate of the reduction
reaction by reducing superoxide anion concentrations. Therefore, the % Inhibition of the reduction reaction
can be determined as a measurement of SOD activity.
Product Use This assay kit is used to evaluate SOD activity in vitro. It is for research use only. Not for use in animals,
humans, or diagnostic procedures.