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NF-kB p65 Antibody
AF5006
NF-kB p65 Antibody
NF-kB p65 Antibody
兔
Human, Mouse, Rat, Monkey
WB, IHC, IF/ICC, ELISA(peptide)
50ul
产品: | NF-kB p65 抗体 |
货号: | AF5006 |
来源: | Rabbit |
应用: | WB, IHC, IF/ICC, ELISA(peptide) |
反应: | Human, Mouse, Rat, Monkey |
预测: | Pig, Bovine, Horse, Sheep, Dog |
分子量: | 65~80kD; 60kD(Calculated). |
蛋白号: | Q04206 |
RRID: | AB_2834847
来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:200, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
反应:
Human,Mouse,Rat,Monkey
预测:
Pig(100%), Bovine(91%), Horse(91%), Sheep(91%), Dog(100%)
克隆:
Polyclonal
特异性:
NF-kB p65 Antibody detects endogenous levels of total NF-kB p65.
RRID:
AB_2834847
引用格式: Affinity Biosciences Cat# AF5006, RRID:AB_2834847.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
1). Huang S et al. Ras guanine nucleotide-releasing protein-4 promotes renal inflammatory injury in type 2 diabetes mellitus. Metabolism 2022 Jun;131:155177. (PubMed: 35218794) [IF=31.373]
2). Li X et al. Upregulation of BCL-2 by acridone derivative through gene promoter i-motif for alleviating liver damage of NAFLD/NASH. Nucleic Acids Res 2020 Sep 4;48(15):8255-8268. (PubMed: 32710621) [IF=19.160]
Application: WB Species: mouse Sample: liver
Figure 7. Effect of A22 on ameliorating apoptosis, ER stress, inflammation, metabolic syndrome, and fibrogenesis in HF diet-fed mice. (A) Effect of A22 on BCL-2 gene transcription. (B) Effect of A22 on BAX gene transcription. (C) Effect of A22 on expressions of apoptosis-related proteins in liver. The extracted proteins from the liver were immunoblotted with specific antibodies, and quantified based on the loading control of ACTIN. (D) Effect of A22 on ER stress. The UPR proteins (IRE-1, PERK, elF-2 and CHOP) were analyzed by using western Blot. (E) Effect of A22 on expressions of inflammatory factors. (F) Effect of A22 on expressions of fibrogenic proteins.
3). Han J et al. YY1 complex promotes Quaking expression via super-enhancer binding during EMT of hepatocellular carcinoma. Cancer Res 2019 Feb 13 (PubMed: 30760518) [IF=13.312]
Application: WB Species: human Sample: PLC-PRF-5 cells
Fig. 1 | YY1 is a key transcription factor for QKI expression in PLC-PRF-5 cells. (g) Co-IP of endogenous YY1, p65, and p300 was performed in PLC-PRF-5 cells.
Application: IHC Species: mouse Sample: tumor tissues
Fig. 5| QKI expression is required for the YY1 complex to promote the metastasis and malignancy of HCC. (a) Comparison of PLC-PRF-5 and Hep3B tumor growth in mice were measured starting 6 days after implantation. All of the groups were euthanized on day 24. (b) Metastatic lung nodules were counted in each group. (c) QKI, p65, YY1, E-cadherin, and Vimentin expression levels in the tumor tissues of p65, YY1, p65+YY1, QKI and p65+YY1+siQKI groups analyzed through IHC staining.
4). Ai XY et al. Phenytoin silver: a new nanocompound for promoting dermal wound healing via comprehensive pharmacological action. Theranostics 2017 Jan 5;7(2):425-435 (PubMed: 28255340) [IF=11.600]
Application: IHC Species: human Sample:
Figure 2. PnAg promotes wound healing in SD rats. (A) Photographs of rat skin full-thickness excision wounds on different post-excision days. (B) Change in wound areas of SD rats after treatment; (C) and (D) Expression levels of collagen I, NF-κB, TGF-ß, MMP-2, and MMP-9 in tissues on day 7 and 17 detected by immunohistochemistry. (E) Histogram of protein expression levels in these tissues. (F) and (G) Histomorphological changes in wound tissues stained by Masson trichrome and HE on day 17.
5). Zhang Z et al. Clearance of apoptotic cells by mesenchymal stem cells contributes to immunosuppression via PGE2. EBioMedicine 2019 Jul;45:341-350 (PubMed: 31248835) [IF=11.205]
6). Li C et al. Oxyberberine, a novel gut microbiota-mediated metabolite of berberine, possesses superior anti-colitis effect: Impact on intestinal epithelial barrier, gut microbiota profile and TLR4-MyD88-NF-κB pathway. Pharmacol Res 2020 Feb;152:104603 (PubMed: 31863867) [IF=10.334]
Application: WB Species: Mice Sample: colonic tissues
Fig. 6. Effect of OBB on the activation of TLR4-MyD88-NF-κB signaling pathway in DSS-induced colonic tissues. (A) Representative Western blotting images of TLR4, MyD88, cytoplasmic p65, nuclear p65, p-IκBα and IκBα. Changes in the relative protein expression levels of TLR4 (B), MyD88 (C), nuclear p65 (D), cytoplasmic p65 (E), and p-IκBα/IκBα ratio (F) were measured. Data are shown as the mean ± SEM (n = 3). # P < 0.05, ## P < 0.01 vs. Control group, * P < 0.05, ** P < 0.01 vs. DSS group.
7). Pan Y et al. The STING antagonist H‐151 ameliorates psoriasis via suppression of STING/NF‐κB‐mediated inflammation. Br J Pharmacol 2021 Aug 30. (PubMed: 34460100) [IF=9.473]
8). Wang N et al. Fibroblast Growth Factor 21 Exerts Its Anti-inflammatory Effects on Multiple Cell Types of Adipose Tissue in Obesity. Obesity (Silver Spring) 2019 Jan 31 (PubMed: 30703283) [IF=9.298]
9). Lu Y et al. Water Extract of Shepherd's Purse Prevents High-Fructose Induced-Liver Injury by Regulating Glucolipid Metabolism and Gut Microbiota. Food Chem 2020 Nov 4;128536. (PubMed: 33189481) [IF=9.231]
Application: WB Species: Mice Sample: liver tissue
Fig. 3. Effects of WESP on glucolipid metabolism, oxidative stress and inflammation in HF-fed mice: (A) Fasting glucose level, (B) Fasting insulin level, (C) HOMA-IR, (D) Serum leptin level, (E) OGTT, oral glucose tolerance test, (F) AUC of OGTT, (G) MDA, (H) SOD, (I) GSH-Px, (G) IL-1, (K) IL-6, (L) TNF-α, (M) Western blot analysis of NF-κB in liver tissue, (N) Relative levels of NF-κB expression.
10). Li Y et al. Interleukin 17A deficiency alleviates fluoride-induced testicular injury by inhibiting the immune response and apoptosis. Chemosphere 2021 Jan;263:128178. (PubMed: 33297146) [IF=8.943]
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