Bovine Methylmalonyl- CoA mutase, mitochondrial, MUT ELISA KIT
Product Name:Bovine Methylmalonyl- CoA mutase, mitochondrial, MUT ELISA KIT
Packing:96T
Catalog No.:ELI-37514b
Gene Name:Bovine Methylmalonyl-CoA mutase, mitochondrial, MUT
Detect Range:3.125-200ng/ml
Sensitivity:1.875ng/ml
Target Protein Name:Bovine Methylmalonyl-CoA mutase, mitochondrial, MUT
Alternative Name:MUT,Bovine Methylmalonyl-CoA mutase, mitochondrial, MUT
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Methylmalonyl- CoA mutase, mitochondrial, MUT ELISA KIT allows for the in vitro quantitative determination of Bovine Methylmalonyl-CoA mutase, mitochondrial, MUT concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Methylmalonyl- CoA mutase, mitochondrial, MUT ELISA KIT has been pre-coated with an Bovine Methylmalonyl-CoA mutase, mitochondrial, MUT antibody specific to Bovine Methylmalonyl-CoA mutase, mitochondrial, MUT .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Methylmalonyl-CoA mutase, mitochondrial, MUT and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Methylmalonyl-CoA mutase, mitochondrial, MUT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Methylmalonyl-CoA mutase, mitochondrial, MUT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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