The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
1:500 - 1:2000
IHC
1:50 - 1:200
Western blot analysis of extracts of various cell lines, using ISG15 antibody.
Immunohistochemistry of paraffin-embedded human gastric cancer using ISG15 Antibody.
Immunohistochemistry of paraffin-embedded rat brain using ISG15 Antibody.
Immunohistochemistry of paraffin-embedded human breast cancer using ISG15 Antibody.
Immunohistochemistry of paraffin-embedded mouse brain using ISG15 Antibody.
Background:
The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted.
Storage:
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3