The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
1:500 - 1:2000
IHC
1:50 - 1:100
IF
1:50 - 1:200
Western blot analysis of extracts of various cell lines, using YAP1 antibody.
Immunohistochemistry of paraffin-embedded human stomach using YAP1 antibody.
Immunofluorescence analysis of HeLa cells using YAP1 antibody.
Immunofluorescence analysis of NIH/3T3 cells using YAP1 antibody.
Immunofluorescence analysis of PC12 cells using YAP1 antibody.
Background:
This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms.
Storage:
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.