The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
1:200 - 1:2000
IHC
1:50 - 1:500
Western blot analysis of extracts of various cell lines, using AK5 antibody.
Background:
This gene encodes a member of the adenylate kinase family, which is involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. This member is related to the UMP/CMP kinase of several species. It is located in the cytosol and expressed exclusively in brain. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene.
Storage:
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.