Plasmid classification: plant series, protein overexpression vector
Plasmid size: 11849bp
Prokaryotic resistance: Kan
Selection marker: Hyg
Clonal strain: DH5 alpha
Culture conditions: 37 C, aerobic LB
Expression host: plant cells
5'sequencing primers: M13F:TGTAAAACGACGGCCAGT
Primers for 3'sequencing: primers designed based on sequences
Expression: Plant
Use: Plant expression
pCAMBIA1301-35SN Decription
This vector contains a fully functional gusA reporter construct for simple and sensitive analysis of gene function or presence in regenerated plants by GUS assay. The construct uses E.coli gusA with an intron (from the castor bean catalase gene) inside the coding sequence to ensure that expression of glucuronidase activity is derived from eukaryotic cells, not from expression by residual A.tumefaciens cells. This vector is suitable for insertion of other genes of interest containing their own promoter and terminator. Researchers can excise the gusA gene and insert their own gene of interest in its place or use these vectors to create fusions of gusA with their gene of interest. These vectors contain the pUC18 polylinker-lacZa.