The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
1:200 - 1:2000
IHC
1:50 - 1:200
Western blot analysis of extracts of various cell lines, using SLC25A1 antibody.
Immunohistochemistry of paraffin-embedded rat brain using SLC25A1 antibody.
Background:
This gene encodes a member of the mitochondrial carrier subfamily of solute carrier proteins. Members of this family include nuclear-encoded transporters that translocate small metabolites across the mitochondrial membrane. This protein regulates the movement of citrate across the inner membranes of the mitochondria. Mutations in this gene have been associated with combined D-2- and L-2-hydroxyglutaric aciduria. Pseudogenes of this gene have been identified on chromosomes 7, 11, 16, and 19. Alternative splicing results in multiple transcript variants.
Storage:
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.