Figure 1. Actin Stress Fibers stained with Acti-stain™ 488 in a Swiss 3T3 cell.
Swiss 3T3 cell stained with anti-vinculin (red),
Dapi (blue nucleus) and F-actin is stained with
Acti-stain™ 488 (green F-actin, Cat.# PHDG1).
Figure 2. Emission and excitation scans for Acti-stain™ phalloidins
Absorbance and fluorescence scan of Acti-stain™ 488.
Labeled phalloidin was diluted into methanol and its
absorbance and excitation spectra were scanned between
350-750 and 500-750 nm, respectively. Absorbance peaks
at 500 nm and fluorescence at 550 nm.