Target Protein Name:55 kDa erythrocyte membrane protein(MPP1
Alternative Name:MPP1,55 kDa erythrocyte membrane protein(MPP1 Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit Product Description:Human 55 kDa erythrocyte membrane protein, MPP1 ELISA KIT allows for the in vitro quantitative determination of 55 kDa erythrocyte membrane protein(MPP1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle: The microtiter plate provided in Human 55 kDa erythrocyte membrane protein, MPP1 ELISA KIT has been pre-coated with an 55 kDa erythrocyte membrane protein(MPP1 antibody specific to 55 kDa erythrocyte membrane protein(MPP1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for 55 kDa erythrocyte membrane protein(MPP1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain 55 kDa erythrocyte membrane protein(MPP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of 55 kDa erythrocyte membrane protein(MPP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!