- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 国产
- ELI-21015h
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Protein kinase C-binding protein 1,ZMYND8
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Protein kinase C- binding protein 1, ZMYND8 ELISA KIT
Product Name:Human Protein kinase C- binding protein 1, ZMYND8 ELISA KIT
Packing:96T
Catalog No.:ELI-21015h
Gene Name:ZMYND8
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Protein kinase C-binding protein 1(ZMYND8
Alternative Name:ZMYND8,Protein kinase C-binding protein 1(ZMYND8
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Protein kinase C- binding protein 1, ZMYND8 ELISA KIT allows for the in vitro quantitative determination of Protein kinase C-binding protein 1(ZMYND8 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Protein kinase C- binding protein 1, ZMYND8 ELISA KIT has been pre-coated with an Protein kinase C-binding protein 1(ZMYND8 antibody specific to Protein kinase C-binding protein 1(ZMYND8 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Protein kinase C-binding protein 1(ZMYND8 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Protein kinase C-binding protein 1(ZMYND8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Protein kinase C-binding protein 1(ZMYND8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Analysis of Protein Kinase C Function
Protein kinase C is a key element in signal transduction, cell regulation, and tumor promotion, influencing cellular responses to many stimuli at a variety of levels, including receptors, transduction systems, ion channels, and membrane pumps
Translocation Assays of Protein Kinase C Activation
The protein kinase C (PKC) family members include at least 11 different isoforms that, based on their different requirements for activation, have been divided into three subfamilies, the Ca2+ -dependent (cPKCα, (βI , βII
PKC系统(protein kinase C system，PKC system)
( diacylglycerol, DAG)和1,4,5-三磷酸肌醇(IP3)。IP3动员细胞内钙库释放Ca2 到细胞质中与钙调蛋白结合，随后参与一系列的反应；而DAG在Ca2 的协同下激活蛋白激酶C(protein kinase C，PKC)，然后通过蛋白激酶C引起级联反应，进行细胞的应答, 故此将该系统称为PKC系统,或称为IP3、DAG、Ca2 信号通路。
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