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武汉华美生物
1.56 pg/mL-100 pg/mL
ELISA
Mus musculus (Mouse)
serum, plasma, cell culture supernates, tissue homogenates, cell lysates
96T
武汉华美具有完善的ELISA试剂盒开发平台,成熟的抗原、抗体研发系统,熟练掌握各种酶联技术,如双抗夹心法、(直接)竞争法、间接竞争法、阻断法、间接法、双抗原夹心法等方法,结合我公司的诊断试剂开发团队我们可以有效的将试剂盒开发为临床诊断级别,质量处于世界前列。
我们的ELISA试剂盒,采用三轮质检方式,最大限度地保证了试剂盒最小的批内差、批间差。试剂盒组分经过重重优化,每个组分的稳定性都完美的经过高温破坏测验,最大程度的避免了长途运输和保存过程中抗原抗体的失活,武汉华美试剂盒凭借其完美的标曲、严格的质控、较高的稳定性,在世界范围内的科研试剂领域中脱颖而出,深受数十个国家上万名使用者的青睐。
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of mouse VEGF-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 85 | ||||
Range % | 80-92 | |||||
1:2 | Average % | 95 | ||||
Range % | 91-105 | |||||
1:4 | Average % | 102 | ||||
Range % | 92-110 | |||||
1:8 | Average % | 90 | ||||
Range % | 86-98 |
The recovery of mouse VEGF-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 94 | 89-98 | ||||
EDTA plasma (n=4) | 96 | 90-100 | ||||
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