背景
TOMM20 (Translocase Of Outer Mitochondrial Membrane 20) is a Protein Coding gene. Diseases associated with TOMM20 include Perry Syndrome. Among its related pathways are Neuroscience and Glucose / Energy Metabolism. Gene Ontology (GO) annotations related to this gene include unfolded protein binding and P-P-bond-hydrolysis-driven protein transmembrane transporter activity. An important paralog of this gene is TOMM20L.
免疫原信息
| 免疫原 | Recombinant fusion protein containing a sequence corresponding to amino acids 20-145 of human TOM20 (NP_055580.1). |
| 序列 | YCIYFDRKRRSDPNFKNRLRERRKKQKLAKERAGLSKLPDLKDAEAVQKFFLEEIQLGEELLAQGEYEKGVDHLTNAIAVCGQPQQLLQVLQQTLPPPVFQMLLTKLPTISQRIVSAQSLAEDDVE |
| 基因ID | 9804 |
| Swiss Prot | Q15388 |
| 别名 | TOMM20; MAS20; MOM19; TOM20; translocase of outer mitochondrial membrane 20 |
应用
| 反应物种 | Human, Mouse, Rat |
| 验证应用 | WBIHCIFIP |
| 推荐稀释比 | | WB | 1:1000 - 1:50000 | | IHC | 1:50 - 1:200 | | IF | 1:50 - 1:200 | | IP | 1:20 - 1:50 | |
| 理论分子量 | 16kDa |
| 实际分子量 | 16kDa |
| 存储缓冲液 | Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% sodium azide,50% glycerol,pH7.3. |
| 实验方法 | Western blotting |
| 阳性样本 | THP-1,HeLa,MCF7,Mouse brain |
| 细胞定位 | Mitochondrion outer membrane,Single-pass membrane protein |
| 纯化方式 | Affinity purification |
TOM20 Monoclonal Antibody 检测图片
Western blot - TOM20 Monoclonal Antibody (A19403)
Western blot analysis of extracts of various cell lines, using TOM20 antibody (A19403) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot - TOM20 Monoclonal Antibody (A19403)
Western blot analysis of extracts of THP-1 cells, using TOM20 antibody (A19403) at 1:1000-1:256000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunohistochemistry - TOM20 Monoclonal Antibody (A19403)
Immunohistochemistry of paraffin-embedded Rat kidney using TOM20 antibody (A19403) at dilution of 1:200 (40x lens).
Immunohistochemistry - TOM20 Monoclonal Antibody (A19403)
Immunohistochemistry of paraffin-embedded Human colon carcinoma using TOM20 antibody (A19403) at dilution of 1:200 (40x lens).
Immunohistochemistry - TOM20 Monoclonal Antibody (A19403)
Immunohistochemistry of paraffin-embedded Mouse heart using TOM20 antibody (A19403) at dilution of 1:200 (40x lens).
Immunofluorescence - TOM20 Monoclonal Antibody (A19403)
Immunofluorescence analysis of H9C2 cells using TOM20 antibody (A19403) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - TOM20 Monoclonal Antibody (A19403)
Immunofluorescence analysis of L929 cells using TOM20 antibody (A19403) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - TOM20 Monoclonal Antibody (A19403)
Immunofluorescence analysis of U2OS cells using TOM20 antibody (A19403) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - TOM20 Monoclonal Antibody (A19403)
Confocal immunofluorescence analysis of HeLa cells using TOM20 antibody (A19403) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation - TOM20 Monoclonal Antibody (A19403)
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug TOM20 antibody (A19403). Western blot was performed from the immunoprecipitate using TOM20 antibody (A19403) at a dilition of 1:1000.
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