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Related Products:
Vascular Cell Basal Medium (ATCC PCS-100-030) Endothelial Cell Growth Kit/BBE (ATCC PCS-100-040) Endothelial Cell Growth Kit/VEGF (ATCC PCS-100-041) Dulbecco's Phosphate Buffered Saline (D-PBS) (ATCC 30-2200) Trypsin-EDTA for Primary Cells (ATCC PCS-999-003) Trypsin Neutralizing Solution (ATCC PCS-999-004) Gentamicin-Amphotericin B Solution (ATCC PCS-000-025) Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002) Phenol Red (ATCC PCS-999-001)
The cells are cryopreserved in the second passage to ensure the highest viability and plating efficiency. ATCC primary pooled HUVECs are derived from 10 individual donors, minimizing the lot-to-lot variability associated with cells derived from single donors. Large lot sizes assure consistency for large-scale studies. A complete solution to propagate pooled HUVECs in low serum conditions with or without the addition of human recombinant VEGF. Use of the Endothelial Cell Growth Kit-VEGF will support a faster rate of proliferation because of the presence of several purified human recombinant (rh) growth factors (rh VEGF, rh EGF, rh FGF basic and rh IGF-1) combined with heparin and hydrocortisone. Use of the Endothelial Cell Growth Kit-BBE, which contains Bovine Brain Extract (BBE), is recommended if a more "authentic," less defined culture medium is desired.