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52 years
上皮样
duct
CRL-4023™
人
0
胰腺
大量
冻存运输
贴壁生长
Designations: | hTERT-HPNE | ||
Depositors: | M Ouellette | ||
Biosafety Level: | 2 [cells containing SV40 viral DNA sequences ] | ||
Shipped: | frozen | ||
Medium & Serum: | See Propagation | ||
Growth Properties: | adherent | ||
Organism: | Homo sapiens | ||
Morphology: | epithelial-like |
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Source: | Organ: pancreas Tissue: duct |
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Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
Restrictions: | This material requires that the Addendum for Commercial and For-Profit Organizations or the Addendum for Noncommercial and Academic Organizations be signed and returned to ATCC before shipment. The price listed above is for noncommercial and academic organizations only. Commercial and for-profit organizations should call for pricing. | ||
Applications: | s part of our quality control, we have tested this cell line for its ability to grow for a minimum of 15 population doublings after recovery from cryopreservation. We have also compared its karyotype, telomerase expression level, growth rate, morphology and tissue-specific markers when first recovered from cryopreservation with that of cells at 10+ population doublings to ensure that there is no change in these parameters and that the cells are capable of extended proliferation. Exposing hTERT-HPNE cells to sodium butyrate and 5-aza-2'-deoxycytidine lead to the formation of pancreatic ductal cells marked by the expression of p-glycoprotein (multidrug resistance (MDR-1)), carbonic anhydrase II, and the cytokeratins 7, 8, and 19. hTERT-HPNE cells were found to have properties of the intermediary cells formed during acinar-to-ductal metaplasia, which included their undifferentiated phenotype, expression of Nestin and, evidence of active Notch signaling [Pubmed: 15924149]. The infected cells became positive for telomerase, failed to senesce, and were still proliferating after more than 150 doublings [Pubmed: 12589817]. |
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Antigen Expression: | Positive for epithelial marker pan-cytokeratin (immunocytochemistry); positive for nestin (flow cytometry). | ||
DNA Profile (STR): | D5S818: 11 D13S317: 12,13 D7S820: 9,10 D16S539: 12,13 vWA: 17 Amelogenin: X,Y TPOX: 8,11 CSF1PO: 12 TH01: 8,9 |
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Cytogenetic Analysis: | This is a pseudodiploid human cell line of male origin with a modal chromosome number of 46 and a low polyploidy rate. Approximately 50% of the cells contained a consistent derivative chromosome 21 with additional material at p12. | ||
Age: | 52 years | ||
Gender: | male | ||
Comments: | The hTERT-HPNE (Human Pancreatic Nestin Expressing cells) cell line was developed from human pancreatic duct by transduction with a retroviral expression vector (pBABEpuro) containing the hTERT gene. The infected cells became positive for telomerase, failed to senesce, and were still proliferating after more than 150 doublings [Pubmed: 12589817].Exposing hTERT-HPNE cells to sodium butyrate and 5-aza-2'-deoxycytidine lead to the formation of pancreatic ductal cells marked by the expression of p-glycoprotein (multidrug resistance (MDR-1)), carbonic anhydrase II, and the cytokeratins 7, 8, and 19. hTERT-HPNE cells were found to have properties of the intermediary cells formed during acinar-to-ductal metaplasia, which included their undifferentiated phenotype, expression of Nestin and, evidence of active Notch signaling [Pubmed: 15924149]. As part of our quality control, we have tested this cell line for its ability to grow for a minimum of 15 population doublings after recovery from cryopreservation. We have also compared its karyotype, telomerase expression level, growth rate, morphology and tissue-specific markers when first recovered from cryopreservation with that of cells at 10+ population doublings to ensure that there is no change in these parameters and that the cells are capable of extended proliferation. |
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Propagation: | ATCC complete growth medium: The base medium for this cell line is:
Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
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Subculturing: | Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
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Preservation: | Freeze medium: 95% FBS; 5% DMSO | ||
Doubling Time: | about 31 hours | ||
References: | 16172577: Lee KM. et al. Notch 2-positive progenitors with the intrinsic ability to give rise to pancreatic ductal cells. Lab. Investigation 85 (8): 1003-1012, 2005. Pubmed: 15924149 16172578: Lee KM. et al. Immortalization with telomerase of the Nestin-positive cells of the human pancreas. Biochem Biophys Res Commun. ; 301(4):1038-44 (2003). Pubmed: 12589817 |
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