大家都在搜
手机验证
人
0
大量
其他细胞类型
冻存运输
20 years
贴壁生长
bronchus
上皮样
肺
囊肿性纤维化
CRL-4017™
Designations: | CuFi-6 | ||
Depositors: | AJ Klingelhutz | ||
Biosafety Level: | 2 | ||
Shipped: | frozen | ||
Medium & Serum: | See Propagation | ||
Growth Properties: | adherent | ||
Organism: | Homo sapiens | ||
Morphology: | epithelial |
||
Source: | Organ: lung Tissue: bronchus Disease: cystic fibrosis Cell Type: epithelial immortalized with hTERT and HPV-16 E6/E7-LXSN |
||
Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
Restrictions: | This material requires that the Addendum for Commercial and For-Profit Organizations or the Addendum for Noncommercial and Academic Organizations be signed and returned to AT CC before shipment. The price listed above is for noncommercial and academic organizations only. Commerci al and for-profit organizations should call for pricing. | ||
Isolation: | Isolation date: 2001 | ||
Applications: | In addition, it has been verified that no gross changes are observed in karyotype and morphology during the first 10 population doublings. As part of our quality control, we have tested this cell line for its ability to grow for a minimum of 15 population doublings after recovery from cryopreservation. Human airway epithelial (HAE) cell line, CuFi-6, was derived from lung of a 20-year-old patient with cystic fibrosis by retroviral infection with HPV-16 E6/E7-LXSN. Consequently, the cells do not undergo growth arrest in cell culture due to exogenous expression of the telomerase and E6/E7 genes. Another hTERT-immortalized cell line derived from cystic fibrosis HAE is available as ATCC CRL-4013 (CuFi-1). |
||
Antigen Expression: | positive for epithelial marker pan-cytokeratin (immunocytochemistry) | ||
DNA Profile (STR): | Amelogenin: XY CSF1PO: 11 D13S317: 12 D16S539: 11,12 D5S818: 9,12 D7S820:8,11 THO1: 6 TPOX: 8,10 vWA: 17,19 |
||
Cytogenetic Analysis: | This is a near-diploid cell line of male origin. The most consistent karyotypic aberrations include trisomy of chromosome 5 and 20, monosomy of chromosome 15 or 16, and loss of the Y chromosome. Additionally, the polyploidy rate may increase slightly at high passage. | ||
Age: | 20 years | ||
Gender: | male | ||
Comments: | Human airway epithelial (HAE) cell line, CuFi-6, was derived from lung of a 20-year-old patient with cystic fibrosis by retroviral infection with HPV-16 E6/E7-LXSN. Consequently, the cells do not undergo growth arrest in cell culture due to exogenous expression of the telomerase and E6/E7 genes. [22566] CuFi-6 cells are homozygous for the delta F508 cystic fibrosis-causing mutation (delta F508/delta F508). [92666] Another hTERT-immortalized cell line derived from cystic fibrosis HAE is available as ATCC CRL-4013 (CuFi-1). As part of our quality control, we have tested this cell line for its ability to grow for a minimum of 15 population doublings after recovery from cryopreservation. In addition, it has been verified that no gross changes are observed in karyotype and morphology during the first 10 population doublings. |
||
Propagation: | ATCC complete growth medium: These cells are grown in a serum-free medium: BEGM (Bronchial Epithelial Growth Medium, Serum-free) from Lonza (BEGM Bullet Kit; CC-3170) made of BEBM basal medium and SingleQuot additives (ATCC does not use gentamycin-amphotericin B) supplemented with 50 �g/ml G-418. Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
||
Subculturing: | Protocol: Note: The culture flasks should be pre-coated with 60µg/ml solution of Human Placental Collagen Type IV. (Sigma, Cat. No. C-7521) at least 18 hours in advance then air-dried and rinsed 2-3 times with Dulbecco's Phosphate Buffered Saline.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended. Medium renewal: every 3 to 4 days |
||
Preservation: | Freeze medium BEGM supplemented with 10% (v/v) DMSO and 30% (v/v) fetal bovine serum Storage temperature: liquid nitrogen vapor phase |
||
Doubling Time: | about 64 hours | ||
Related Products: | 0.25% (w/v) Trypsin - 0.53mM EDTA in Hank's BSS (w/o Ca++, Mg ++): ATCC 30-2101 Recommended serum: ATCC 30-2020 Phosphate-buffered saline: ATCC 30-2200 Cell culture tested DMSO: ATCC 4-X |
||
References: | 22566: Halbert CL, et al. The E7 gene of human papillomavirus type 16 is sufficient for immortalization of human epithelial cells. J. Virol. 65: 473-478, 1991. PubMed: 1845902 47354: Bodnar AG, et al. Extension of life-span by introduction of telomerase into normal human cells. Science 279: 349-352, 1998. PubMed: 9454332 92666: Zabner J, et al. Development of cystic fibrosis and noncystic fibrosis airway cell lines . Am. J. Physiol. Lung Cell Mol Physiol. 284: L844-L854, 2003. PubMed: 12676769 92667: Kiyono T, et al. Both Rb/p161NK4a inactivation and telomerase activity are required to immortalize human epithelial cells. Nature 396: 84-88, 1998. PubMed: 9817205 |
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。