blackPREP FFPE DNA Kit
福尔马林固定石蜡包埋的组织样本的DNA试剂盒
产品优势
★分子病理的样本,包含FFPE(新鲜、5年或10年以上的样本)和穿刺样本,都可适用
★无需二甲苯脱蜡,直接裂解
★操作简单,无需太多的人工操作,整个流程下来仅需2.5个小时
订货信息
货号:845-BP-0021010(blackPREP FFPE DNA Kit - 10 reactions)
货号:845-BP-0021050(blackPREP FFPE DNA Kit - 50 reactions)
货号:845-BP-0021250(blackPREP FFPE DNA Kit - 250 reactions)
适用于各种福尔马林固定和石蜡包埋的组织样本。
应用实例
FFPE样本提取试剂盒 blackRPEP FFPE DNA Kit
案例一、上海某中心医院提取2年、7年和8年的大肠癌切片样本
背景:该医院的转化医学院,有大量的病理切片需要进行核酸提取,下游做二代测序和芯片。
样本:大肠癌病理切片,分别为2014年样本2个,2009年样本1个,2008年样本1个
方法:按照说明书操作,其中2014年样本洗脱体积100微升,2009年和2008年样本洗脱体积50微升。
德国耶拿ScanDrop检测结果:
样品 |
浓度 ng/ul |
260/280 |
260/230 |
1-2014 |
2695.8 |
1.92 |
2.06 |
2-2014 |
1360.1 |
1.96 |
2.13 |
3-2009 |
1104.9 |
1.94 |
2.06 |
4-2008 |
581.1 |
1.99 |
2.13 |
案例二、某第三方检测公司提取10年病理切片
背景:此公司为确保客户提供的原始样本提取得到的核酸质量足够好,故采用国外原装进口的试剂盒进行核酸提取,满足下游芯片、NGS等实验要求。
实验样本:1和2号为胃肠腺癌,3号为胃腺癌,4和5号为平滑肌,其中3和4属于微量样本(以上均为10年以上样本)
方法:按照说明书操作,洗脱体积为50微升
德国耶拿ScanDrop检测结果
|
浓度 ng/ul |
260/280 |
260/230 |
1 |
1038.5 |
1.96 |
2.12 |
2 |
908.9 |
1.96 |
2.09 |
3 |
127.4 |
1.92 |
1.9 |
4 |
53.3 |
1.93 |
1.63 |
5 |
257.8 |
1.98 |
2.07 |
Product Details
- Extra short lysis time by using special Lysis Buffer
- No deparaffinization is needed
- Without use of hazardous or toxic components like phenol, octane or xylene
- High yield and optimal quality in less than 2.5 h
- Tested for different amount of paraffin and tissue
- Suitable for many kinds of tissue samples
- High quality nucleic acids for direct use or storage
- Excellent DNA integrity
The blackPREP FFPE DNA Kit has been designed as a very efficient tool for fast isolation of genomic DNA from FFPE samples for subsequent purposes. The kit can be used with different amount of FFPE tissue samples / sections, up to a maximum of 50 mg. The extraction procedure is based on a new patented chemistry and combines lysis of FFPE tissue samples with subsequent binding of nucleic acids onto the surface of a Spin Filter membrane. Thereby the optimized procedure renders an extra step of deparaffinization and the usage of toxic solvents (e.g. xyolol or octane) unnecessary. After several washing steps the nucleic acids are eluted from the membrane by using Elution Buffer. The extraction is optimized to get maximum of yield for immediate downstream applications and storage for future use.
Specifications
Starting material
- FFPE (formalin fixed paraffin embedded) tissue samples
- Maximum amount of tissue: 50 mg
Average yield
Depends on the type and starting quantity of the FFPE tissue material
Extraction time
Approx. 2.5 hours (incl. lysis)
Binding capacity
> 50 μg genomic DNA
Average purity
1,7 – 2,2
Order Information
Order number |
Quantity |
845-BP-0021010 |
10 reactions |
845-BP-0021050 |
50 reactions |
845-BP-0021250 |
250 reactions |