ExKine™ 细胞核蛋白提取试剂盒

ExKine™ 细胞核蛋白提取试剂盒 (KTP3002)

产品核心参数

• 应用领域：哺乳动物细胞/组织核蛋白提取与染色
• 产品中心：细胞分析 › 细胞器处理系列
• 下游兼容性：Western Blot｜酶活力测定｜EMSA｜报告基因实验

技术优势

• 双区室精准分离 - 通过CES/NES双相溶液体系实现细胞浆与核蛋白的物理分离，交叉污染率<3%
• 活性保留技术 - 非变性提取方案确保DNA结合蛋白功能完整性
• 即用型配方 - 预混DTT与蛋白酶抑制剂，单批次生产确保组分稳定性

试剂盒组分

组分名称	保存条件	单次用量
细胞浆蛋白溶液A (CES A)	2-8°C	500μL/测试
细胞浆蛋白溶液B (CES B)	2-8°C	200μL/测试
核提取溶液 (NES)	避光2-8°C	1mL/测试
DTT (500X)	-20°C	2μL/测试
蛋白酶抑制剂 (100X)	-20°C	10μL/测试

订购信息

• 50次装 | ¥316 (库存代码：KTP3002-50) 当日发货
• 200次装 | ¥816 (库存代码：KTP3002-200) 次日发货

操作规范

• 全程2-8°C低温操作，建议使用预冷离心机(≥12,000×g)
• 组织样本需预先进行机械粉碎处理
• 提取后的核蛋白建议-80°C分装保存

重要提示：本产品含蛋白酶抑制剂AEBSF，避免接触皮肤。
适用样本量为1×10⁶-5×10⁷个细胞或50-100mg组织

*产品开发文献索引：Nucleic Acids Res. 2021;49(9):e52｜Nat Protoc. 2020;15(3):1188-1207

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

ExKine™ Nuclear Protein Extraction Kit (KTP3002)

Key Product Specifications

• Application: Mammalian cell/tissue nuclear protein extraction and staining
• Product Category: Cell Analysis › Organelle Processing Series
• Downstream Compatibility: Western Blot｜Enzyme Activity Assay｜EMSA｜Reporter Gene Assay

Technical Advantages

• Dual-Compartment Precision Separation - Physical separation of cytoplasmic and nuclear proteins via CES/NES biphasic solution system, with cross-contamination rate <3%
• Activity Preservation Technology - Non-denaturing extraction protocol maintains functional integrity of DNA-binding proteins
• Ready-to-Use Formulation - Pre-mixed DTT and protease inhibitors, with single-batch production ensuring component stability

Kit Components

Component	Storage Condition	Single Use Volume
Cytoplasmic Extraction Solution A (CES A)	2-8°C	500μL/test
Cytoplasmic Extraction Solution B (CES B)	2-8°C	200μL/test
Nuclear Extraction Solution (NES)	Light-protected 2-8°C	1mL/test
DTT (500X)	-20°C	2μL/test
Protease Inhibitor Cocktail (100X)	-20°C	10μL/test

Ordering Information

• 50 tests | ¥316 (Catalog No.: KTP3002-50) Same-day shipping
• 200 tests | ¥816 (Catalog No.: KTP3002-200) Next-day shipping

Operational Guidelines

• Maintain 2-8°C throughout procedure; use pre-cooled centrifuge (≥12,000×g)
• Tissue samples require mechanical homogenization pretreatment
• Extracted nuclear proteins recommended for aliquot storage at -80°C

Important Note: This product contains AEBSF protease inhibitor—avoid skin contact.
Recommended sample size: 1×10⁶-5×10⁷ cells or 50-100mg tissue

*Development references: Nucleic Acids Res. 2021;49(9):e52｜Nat Protoc. 2020;15(3):1188-1207

Note: Above information may change due to product optimization. Please contact Yakeyin Company for updated specifications.