ALL CELL QUESTION IN ONE SHOT 细工生物 培养基 500ml 价格按常规报价 胎牛血清 25ml X 2 价格600元 胰酶 100ml 价格90元 广谱杀菌试剂 1ml 价格400元 两性霉素B 1ML 价格300元 推荐:保种体系试剂 总价:1390元 该套体系适用 细胞系:乳腺癌类,肝癌类,ATCC来源等正规库来源细胞 细胞来源清晰,用的都是进口试剂耗材,操作老师具有两年以上操作经验,细胞生长变慢,或值得救助的细胞(经广谱杀菌处理后),可用ONE SHOT体系比较培养,并保种,没有特殊添加其他,只是与正规库体系保持一致常年筛选统计后推广。 原代细胞:经优级两性霉素B处理后(普通污染快速消失),在用ONE SHOT体系,细胞平稳性好。新手适合防止污染,增加培养信心很重要。 注:建议以上试剂耗材均原装进口,细胞来源清晰。
Black spots, slow growth, and pain points in organoids - (Black spots combined with our broad-spectrum sterilization solution are relatively complete)
The growth rate is slow. After careful analysis, the proposed plan is to cultivate in imported bottles (Corning, nunc, all German made fine workers are in stock), use seed bank grade reagents (more than 3000 fetal cows, our company has verified in South America for more than five years), use our company's culture medium or GIBCO culture medium, leave enough culture medium (more is okay) for a few days without moving, and check the recovery situation. Combined with our company's trypsin (non-destructive digestion), it should be able to recover to a better state after several generations of transmission, This method is also suitable for the situation where cells crawl after several generations of cell culture (the above method is also suitable for the situation where cells crawl after cell culture and passage, there are many reasons for this factor, and specific situations will be analyzed collectively). This method also suggests that the first generation of primary culture (organoid) should be replaced by ordinary culture medium and fetal bovine (factors are expensive, lag phase is long, and there is no serum instability point), and can be used as a reference when there are enough cells or stable cells in the absence of serum. U-shaped low consumables for organoid matching (without auxiliary conditions for ball forming) and magnetic bead matching for magnetic ball forming can be sent to request information and technical discussion. Email contact
Pain points of organoids
Resuscitation is still possible, passing several generations to three generations will be over, and the longer it gets, the less it will be - there is a good optimization plan for fine craftsmanship revival, and there are picky cells for cryopreservation to discuss later.
Poor condition, daily replacement, high factor consumption, high cost, no improvement, but unfortunately, the cultivation is still missing. The above feasibility is high and stable, and conventional cultivation methods are also familiar.
At first, I was quite satisfied and passed down well. After a few generations, I committed suicide collectively-- Stable and reliable handling is very important.
After the formation of spheroids in organoids, maintaining culture can also lead to the appearance of black spots in cells. This step can also serve as a reference when considering the black spots in cell lines. Keywords include blow molding, seed bank level of the culture system, cryopreservation and recovery, etc. It is our company's solution to solve the problem of slow growth in cell lines and make some technical reserves. Additionally, it can also serve as a reference for organoids. Reagent consumables and cells have all reached the seed bank level. It is recommended to keep them alive and make some technical reserves for difficult problems. Details of issues during training can be communicated in detail.
Our company's reagents and consumables have high cost, high self-discipline, and high cost. In order to maintain seed preservation, the seed bank system is stable and feasible, and can only increase the price by 30%. We also have sufficient technical reserves for the pain points and difficulties of docking organs (ex vivo treatment, digestion, cultivation, maintenance, cryopreservation and recovery, etc.). We have a variety of difficult to cultivate cell lines for more than ten years, and the cost reduction is almost zero. We do not buy products, and the responsibility is greater. The feasibility of solving problems is the hard truth.
20231206: Pain point application expansion, seed bank, recovery of poor cell status after transfection, recovery of drug-resistant strains, primary application, stem cell application, such as bone research requiring long-term culture, tumor formation not ideal strain backup plan, cells requiring long-term culture, slow operation, hypoxia culture, slow differentiation or avoidance of differentiation, and exploration of long-term culture after differentiation
Long term statistics show caution. Difficult to cultivate strains have mostly the same passage time as the primary generation, and cell line black spots and organoid long-term culture black spots have similar characteristics. Therefore, with the above problem-solving ideas and supporting solutions as a reference, several cases have been applied and solved, which is very reasonable
The recommended solution for fine craftsmanship is to import bottle culture (Corning, nunc, all German fine craftsmanship in stock), seed bank grade reagents (more than 3000 fetal cows, our company has verified in South America for more than five years), our company's culture medium or GIBCO culture medium, and leave enough culture medium (more is okay) for a few days to see the recovery situation. Combined with our pancreatic enzyme (non-destructive digestion), several generations should be able to recover the good condition, This method is also suitable for the situation where cells crawl after several generations of cell culture (the above method is also suitable for the situation where cells crawl after cell culture and passage, there are many reasons for this factor, and specific situations will be analyzed collectively). This method also suggests that the first generation of primary culture (organoid) should be replaced by ordinary culture medium and fetal bovine (factors are expensive, lag phase is long, and there is no serum instability point), and can be used as a reference when there are enough cells or stable cells in the absence of serum. U-shaped low consumables for organoid matching (without auxiliary conditions for ball forming) and magnetic bead matching for magnetic ball forming can be sent to request information and technical discussion. Email contact
Recommended target audience:
Screening of Domestic Reagent Consumables for Conservation - Comprehensive Problem Statistics and Technical Reserve of Seed Bank Reagent Consumables Technology
Statistical analysis and technical reserves of one-stop problems in long-term cell culture for clinical specialists
Based on the current characteristics of domestic cultivation, problem statistics and more technical reserves, annual evaluation of domestic reagents and consumables, and problem-solving (black spots, slow growth, preservation after transfection, preservation after drug resistance, difficult to transform cell immunity, primary generation)
Long term statistics are cautious, as difficult to cultivate strains have mostly the same passage time as primary culture. Cell line black spots and organoid black spots are relatively easier to cultivate in primary culture. All of them come from year-round statistics, so there are solutions and supporting solutions to the above problems for reference
Reference for unstable tumor formation group, with a few technical teachers referring to the first issue
Bottle, slow cultivation, slow passage, slow growth, non-invasive pancreatic enzyme, low carbon dioxide, slow growth, slow differentiation, reason: from stem and ball culture, the statistical pattern of pain points in difficult to cultivate cell lines is similar. Divide a batch of evaluations for a simpler operation and provide a set of backup conditions for the unstable tumor formation group. From the perspective of the characteristics of the tumor formation, there are significant advantages, and a set of backup technology reserves is also provided.
20231217:Summary at the end of the year: From the perspective of pain points, the serum-free plan is very difficult. Considering the characteristics of domestic production and research, Seiko recommends a serum-containing plan (with liver, pancreatic, and intestinal types) for about five years of cooperative laboratory experience, starting with 2D and completing the project. There is already a magnetic bead assisted magnetic frame for ball formation, which is highly feasible. The research on chips related to Southeast University and the clear purpose (user reference), principle, purpose, and purpose are all very clear. Next year, Seiko will focus on solving the primary recovery survival rate in DMSO traditional cryopreservation recovery. Currently, the primary PBMC has achieved a high survival rate and will be further optimized. The focus of relevant culture statistics is on cell membrane extraction and macrophage culture differentiation control. Interested teachers can contact us by email.
20231218:Key points for immune cell transfection craftsmanship reference
The key point is to solve cell aggregation. Before the transformation, we recommend Optimized Trypsin, which has an advantage that is not ordinary. Solving cell aggregation is a key point for immune cells. Using this product for no reason is because it does not damage cells or membranes. For specific information, please refer to the product introduction, which is based on case studies over six years.
The key point reversal method is recommended after we have found that the transfection effect of major brands is good. Below are the relevant manufacturers and product numbers. Our company's auxiliary anchor for this type of product is a wall promoting reagent. This product has been developed and applied for five years (industrial virus production, class 293), and immunocyte confocal microscopy is also acceptable. The following is a connection explanation.
The key point is to improve the virus titer. Please refer to the following introduction to enhance the attachment reagent link. Improving the virus titer is crucial. Currently, users related to glioblastoma may have reached the Chinese standard (based on order quantity, it will be applied in five years, and user cases will not be discussed in this section. The technical section can be used)
Key points, breakthrough reasons, and two related auxiliary brief introductions
Optimization of cryopreservation and recovery, DMSO method, improvement of viability, cell quantity and quality. Currently, the conservative introduction of PBMC viability rate of 80% in clinical cooperative laboratories aims to achieve a higher level as much as possible. It is recommended to use adhesive reagent case 293 for verification, which is more reliable. Many details need to be communicated, so that we can produce fewer parameters. The application of immune cells should be much easier.
We specify reagents and consumables, imported consumables, and precision reagents.
The control of growth and differentiation through macrophage culture, membrane extraction, and transfection are currently only in the theoretical stage, and implementation will take a long time. There is involvement in the transfection part, which will be discussed through communication.
Immunological primary culture, establishment of lineages, organoids, etc. If there are related studies, please write to us for clarification. For more information, please contact us via email.