CHO无血清培养液

Product Description:
A serum free media intended for growth of various CHO lines including transfected cells. The CHO-200 is a media optimized for production and contains advanced additives that slow down the cell growth while promoting the protein production.
Designed specifically for production in suspension culture.
Instructions For Use:
Importace: The cell viability should be over 90% and the cells should be in mid-logarithmic phase of growth at the time of serum free media.
Adaptation Of Cells To CHO Serum-free Media :
Direct adaptation:
1. In most cases it is possible to change the cells directly from the serum containing media to serum free media under normal culture conditions.
2. In most cases it is possible to seed CHO cells that have been removed from freezing medium directly in the serum-free medium, when the cell concentration is at least 5 x 10⁵ cells per 23cm². The cells will begin to grow and after a few passages the adaption will be complete.
Note:
However, there are cases where the cells do not adapt successfully after direct transfer and it is necessary to perform gradual adaptation. The cells should be seeded at 3 x 10⁵ per mL in a mix of serum-free medium CHO-200(1:1,V/V) and serum containing media. The serum containing media is reduced to 25% after 2 passages and then cells are grown in serum free media. In order to save time, we recommend paralled experiments with direct adaption and with weaning. Normally, after the second transfer, it will be obvious whether direct adaption has been successful, and if not, only the weaning experiments are continued.
FORM: Liquid, Sterile, Ready to use.
SHEIF LIFE: Maximum 18 months.
STORAGE TEMPERATURE: STORE AT 2 TO 8℃.
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