α-突触核蛋白，Alpha Synuclein Protei

Alpha Synuclein Protein

α-突触核蛋白

α-突触核蛋白是一种在中枢神经系统突触前及核周表达的可溶性蛋白质，它的形态结构多样，有单体、寡聚体、原纤维和纤维等。在病理状态下这些单体会聚集形成不溶性蛋白沉淀，最终导致神经细胞的死亡。α-突触核蛋白与帕金森病的发病机制和相关功能障碍密切相关，是路易小体（Lewy body）的主要成分。


        下面图片展示了α-突触核蛋白的形成过程，这个活性聚集体又被称为“种子”，因为它有催化突触核蛋白单体聚集形成活性聚集体的作用。突触核蛋白单体在正常人体内就存在着，但是不具有致病性，而在帕金森病人的大脑中这些单体聚集成了突触核蛋白聚集体，连同其他一些蛋白形成了路易小体，沉淀在大脑中，使脑细胞死亡。






        目前市面上销售的突触核蛋白都是非活性的，无法作为“种子”来聚集更多的单体，而StressMarq历经三年潜心研发，我们的活性聚集体可以模拟帕金森疾病模型，助力帕金森疾病的研究。下图所示为原代大鼠海马神经元的免疫细胞染色，A-C为对照聚集体处理的，D-F为活性聚集体处理的，绿色的是识别的活性聚集体上的磷酸化丝氨酸129（磷酸化丝氨酸129是活性聚集体的标志）。可以明显看到经过活性突触核蛋白（SPR-322）处理的神经元有明显的路易小体形成。




活性突触核蛋白以及对照蛋白单体和聚合体
 

 
 

活性突触核蛋白 (α-Syn) 聚合体 (SPR-322) 催化活性单体(SPR-321)形成新的聚合体. 硫黄素 T 是一种荧光染料, 可以绑定富含beta折叠的结构, 例如 α-Syn 聚合体. 绑定之后, 硫黄素T光谱会发生红移, 荧光强度会增强. 左侧硫黄素 T 发射光曲线展示了四种试验对象随时间增强的荧光强度(相关于α-Syn 蛋白聚合体),其中 10 nM 活性 α-Syn 聚合体(SPR-322) 与100 μm 活性 α-Syn 单体 (SPR-321) 混合物荧光增强最明显, 另外几组对比分别是活性 α-Syn 聚合物 (SPR-322) 、活性 α-Syn 单体 (SPR-321) 以及 Thioflavin T λex = 450 nm, λem = 485 nm.

 


以下列表为我们目前提供的突触核蛋白产品：

 

产品名称	应用	活性	蛋白来源	货号	规格
α-突触核蛋白单体  人重组蛋白单体 (对照，非活性)	WB, SDS-PAGE, 体内/体外试验		E. coli	SPR-316B SPR-316C SPR-316E	100 μg 200 μg 500 μg
α-突触核蛋白聚合体           NEW 人重组蛋白聚合体 (对照，非活性)	WB, SDS-PAGE, 体内/体外试验		E. coli	SPR-317B SPR-317C SPR-317E	100 μg 200 μg 500 μg
α-突触核蛋白单体              NEW  活性人重组蛋白单体	WB, SDS-PAGE, 体内/体外试验	ACTIVE	E. coli	SPR-321B SPR-321C SPR-321E	100 μg 2x100 μg 5x100 μg
α-突触核蛋白聚合体           NEW  活性人重组蛋白聚合体	WB, SDS-PAGE, 体内/体外试验	ACTIVE	E. coli	SPR-322B SPR-322C SPR-322E	100 μg 2x100 μg 5x100 μg

除了活性α-突触核蛋白之外，我们还有相关抗体：



 

货号	名称	应用	适用物种	特异性
SMC-530D	Alpha Synuclein 抗体	WB | DB | ICC/IF | ELISA	人 | 小鼠 | 大鼠	通过WB和ELISA验证，该抗体与人α突触核蛋白81-130 号氨基酸区域结合. 检测到~14 kDa 的 Alpha synuclein. ~30 kDa 条带是二聚体。
SMC-531D	Alpha Synuclein 抗体	WB | DB | ICC/IF | ELISA	人 | 小鼠 | 大鼠	通过Dot blot和ELISA验证，该抗体与人α突触核蛋白101-130 号氨基酸区域结合. 检测到~14 kDa 的 Alpha synuclein. ~40 kDa 条带是三聚体。
SMC-532D	Alpha Synuclein 抗体	WB | DB | IHC | ICC/IF | ELISA	人 | 小鼠 | 大鼠	通过WB和ELISA验证，该抗体与人α突触核蛋白101-140号氨基酸区域结合. 检测到~14 kDa 的 Alpha synuclein.
SMC-533D	Alpha Synuclein 抗体	WB | DB | IHC | ICC/IF | ELISA	人 | 小鼠 | 大鼠	通过Dot blot和ELISA验证，该抗体与人α突触核蛋白多处区域结合：21-70 aa, 61-90 aa, 和101-140 aa. 检测到~14 kDa 的 Alpha synuclein. ~40 kDa 条带是三聚体。
SPC-742D	Alpha Synuclein 抗体 (pSer129)	WB | ELISA | IHC	人 | 小鼠 | 大鼠	识别α突触核蛋白丝氨酸129位磷酸化的蛋白. 不识别丝氨酸129未磷酸化的突触核蛋白. 检测到100, 75, 45, 15 kDa条带. 15 kDa 以上的条带是寡聚体.

Product Name	Alpha Synuclein Protein
Description	Active Human Recombinant Alpha Synuclein Protein Monomer
Applications	WB, SDS-PAGE, In vivo assay, In vitro assay
Concentration	Lot/batch specific. See included datasheet.
Conjugates	No tag
Nature	Recombinant
Species	Human
Expression System	E. coli
Amino Acid Sequence	MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVATVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGSIAAATGFVKKDQLGKNEEGAPQEGILEDMPVDPDNEAYEMPSEEGYQDYEPEA
Purity	>95%
Protein Length	Full Length
Biological Activity	100 µM alpha synuclein protein monomer (SPR-321) seeded with 10 nM alpha synuclein protein pre-formed fibrils (SPR-322) in 25 µM Thioflavin T (PBS pH 7.4, 100 µl reaction volume) generated a fluorescence intensity of 13,000 Relative Fluorescence Units after incubation at 37°C with shaking at 600 rpm for 24 hours. Fluorescence was measured by excitation at 450 nm and emission at 485 nm on a Molecular Devices Gemini XPS microplate reader.
Field of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Storage Buffer	PBS pH 7.4
Storage Temperature	-80ºC
Shipping Temperature	Dry Ice. Shipping note: Product will be shipped separately from other products purchased in the same order.
Purification	Ion-exchange Purified
Specificity	~14.46 kDa
Cite This Product	Human Recombinant Alpha Synuclein Protein (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SPR-321)
Certificate of Analysis	Certified >95% pure using SDS-PAGE analysis.

Alternative Names	Active Alpha synuclein monomer, Active Alpha-synuclein monomer, Active Alpha synuclein protein monomer, Active Alpha synuclein monomer, Active Alpha-synuclein protein, Non-A beta component of AD amyloid protein, Non-A4 component of amyloid precursor protein, NACP protein, SNCA protein, NACP protein, PARK1 protein, Active Alpha synuclein monomers, SYN protein, Parkinson's disease familial 1 Protein
Research Areas	Alzheimer's Disease, Neurodegeneration, Neuroscience, Parkinson's Disease
Cellular Localization	Cytoplasm, Membrane, Nucleus
Accession Number	NP_000336.1
Gene ID	6622
Swiss Prot	P37840
Scientific Background	Alpha-Synuclein (SNCA) is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals (1). Alpha-synuclein is highly expressed in the mitochondria of the olfactory bulb, hippocampus, striatum and thalamus (2). Functionally, it has been shown to significantly interact with tubulin (3), and may serve as a potential microtubule-associated protein. It has also been found to be essential for normal development of the cognitive functions; inactivation may lead to impaired spatial learning and working memory (4). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimers disease amyloid plaque, and a major component of Lewy body inclusions, and Parkinson’s disease. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin (5, 6).
References	1. “Genetics Home Reference: SNCA”. US National Library of Medicine. (2013). 2. Zhang L., et al. (2008) Brain Res. 1244: 40-52. 3. Alim M.A., et al. (2002) J Biol Chem. 277(3): 2112-2117. 4. Kokhan V.S., Afanasyeva M.A., Van’kin G. (2012) Behav. Brain. Res. 231(1): 226-230. 5. Spillantini M.G., et al. (1997) Nature. 388(6645): 839-840. 6. Mezey E., et al. (1998) Nat Med. 4(7): 755-757.