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α-突触核蛋白，Alpha S
ynuclein ProteinAlpha Synuclein Protein
100ug
 |
活性突触核蛋白 (α-Syn) 聚合体 (SPR-322) 催化活性单体(SPR-321)形成新的聚合体. 硫黄素 T 是一种荧光染料, 可以绑定富含beta折叠的结构, 例如 α-Syn 聚合体. 绑定之后, 硫黄素T光谱会发生红移, 荧光强度会增强. 左侧硫黄素 T 发射光曲线展示了四种试验对象随时间增强的荧光强度(相关于α-Syn 蛋白聚合体),其中 10 nM 活性 α-Syn 聚合体(SPR-322) 与100 μm 活性 α-Syn 单体 (SPR-321) 混合物荧光增强最明显, 另外几组对比分别是活性 α-Syn 聚合物 (SPR-322) 、活性 α-Syn 单体 (SPR-321) 以及 Thioflavin T λex = 450 nm, λem = 485 nm. |
| 产品名称 | 应用 | 活性 | 蛋白来源 | 货号 | 规格 |
| α-突触核蛋白单体 人重组蛋白单体 (对照，非活性) |
WB, SDS-PAGE, 体内/体外试验 | E. coli | SPR-316B SPR-316C SPR-316E |
100 μg 200 μg 500 μg |
|
| α-突触核蛋白聚合体 NEW 人重组蛋白聚合体 (对照，非活性) |
WB, SDS-PAGE, 体内/体外试验 | E. coli | SPR-317B SPR-317C SPR-317E |
100 μg 200 μg 500 μg |
|
| α-突触核蛋白单体 NEW 活性人重组蛋白单体 |
WB, SDS-PAGE, 体内/体外试验 | ACTIVE | E. coli | SPR-321B SPR-321C SPR-321E |
100 μg 2x100 μg 5x100 μg |
| α-突触核蛋白聚合体 NEW 活性人重组蛋白聚合体 |
WB, SDS-PAGE, 体内/体外试验 | ACTIVE | E. coli | SPR-322B SPR-322C SPR-322E |
100 μg 2x100 μg 5x100 μg |
| 货号 | 名称 | 应用 | 适用物种 | 特异性 |
| SMC-530D | Alpha Synuclein 抗体 | WB | DB | ICC/IF | ELISA | 人 | 小鼠 | 大鼠 | 通过WB和ELISA验证，该抗体与人α突触核蛋白81-130 号氨基酸区域结合. 检测到~14 kDa 的 Alpha synuclein. ~30 kDa 条带是二聚体。 |
| SMC-531D | Alpha Synuclein 抗体 | WB | DB | ICC/IF | ELISA | 人 | 小鼠 | 大鼠 | 通过Dot blot和ELISA验证，该抗体与人α突触核蛋白101-130 号氨基酸区域结合. 检测到~14 kDa 的 Alpha synuclein. ~40 kDa 条带是三聚体。 |
| SMC-532D | Alpha Synuclein 抗体 | WB | DB | IHC | ICC/IF | ELISA | 人 | 小鼠 | 大鼠 | 通过WB和ELISA验证，该抗体与人α突触核蛋白101-140号氨基酸区域结合. 检测到~14 kDa 的 Alpha synuclein. |
| SMC-533D | Alpha Synuclein 抗体 | WB | DB | IHC | ICC/IF | ELISA | 人 | 小鼠 | 大鼠 | 通过Dot blot和ELISA验证，该抗体与人α突触核蛋白多处区域结合：21-70 aa, 61-90 aa, 和101-140 aa. 检测到~14 kDa 的 Alpha synuclein. ~40 kDa 条带是三聚体。 |
| SPC-742D | Alpha Synuclein 抗体 (pSer129) | WB | ELISA | IHC | 人 | 小鼠 | 大鼠 | 识别α突触核蛋白丝氨酸129位磷酸化的蛋白. 不识别丝氨酸129未磷酸化的突触核蛋白. 检测到100, 75, 45, 15 kDa条带. 15 kDa 以上的条带是寡聚体. |
| Product Name | Alpha Synuclein Protein |
| Description | Active Human Recombinant Alpha Synuclein Protein Monomer |
| Applications | WB, SDS-PAGE, In vivo assay, In vitro assay |
| Concentration | Lot/batch specific. See included datasheet. |
| Conjugates | No tag
|
| Nature | Recombinant |
| Species | Human |
| Expression System | E. coli |
| Amino Acid Sequence | MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVATVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGSIAAATGFVKKDQLGKNEEGAPQEGILEDMPVDPDNEAYEMPSEEGYQDYEPEA |
| Purity | >95% |
| Protein Length | Full Length |
| Biological Activity | 100 µM alpha synuclein protein monomer (SPR-321) seeded with 10 nM alpha synuclein protein pre-formed fibrils (SPR-322) in 25 µM Thioflavin T (PBS pH 7.4, 100 µl reaction volume) generated a fluorescence intensity of 13,000 Relative Fluorescence Units after incubation at 37°C with shaking at 600 rpm for 24 hours. Fluorescence was measured by excitation at 450 nm and emission at 485 nm on a Molecular Devices Gemini XPS microplate reader. |
| Field of Use | Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only. |
| Storage Buffer | PBS pH 7.4 |
| Storage Temperature | -80ºC |
| Shipping Temperature | Dry Ice. Shipping note: Product will be shipped separately from other products purchased in the same order. |
| Purification | Ion-exchange Purified |
| Specificity | ~14.46 kDa |
| Cite This Product | Human Recombinant Alpha Synuclein Protein (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SPR-321) |
| Certificate of Analysis | Certified >95% pure using SDS-PAGE analysis. |
| Alternative Names | Active Alpha synuclein monomer, Active Alpha-synuclein monomer, Active Alpha synuclein protein monomer, Active Alpha synuclein monomer, Active Alpha-synuclein protein, Non-A beta component of AD amyloid protein, Non-A4 component of amyloid precursor protein, NACP protein, SNCA protein, NACP protein, PARK1 protein, Active Alpha synuclein monomers, SYN protein, Parkinson's disease familial 1 Protein |
| Research Areas | Alzheimer's Disease, Neurodegeneration, Neuroscience, Parkinson's Disease |
| Cellular Localization | Cytoplasm, Membrane, Nucleus |
| Accession Number | NP_000336.1 |
| Gene ID | 6622 |
| Swiss Prot | P37840 |
| Scientific Background | Alpha-Synuclein (SNCA) is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals (1). Alpha-synuclein is highly expressed in the mitochondria of the olfactory bulb, hippocampus, striatum and thalamus (2). Functionally, it has been shown to significantly interact with tubulin (3), and may serve as a potential microtubule-associated protein. It has also been found to be essential for normal development of the cognitive functions; inactivation may lead to impaired spatial learning and working memory (4). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimers disease amyloid plaque, and a major component of Lewy body inclusions, and Parkinson’s disease. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin (5, 6). |
| References | 1. “Genetics Home Reference: SNCA”. US National Library of Medicine. (2013). 2. Zhang L., et al. (2008) Brain Res. 1244: 40-52. 3. Alim M.A., et al. (2002) J Biol Chem. 277(3): 2112-2117. 4. Kokhan V.S., Afanasyeva M.A., Van’kin G. (2012) Behav. Brain. Res. 231(1): 226-230. 5. Spillantini M.G., et al. (1997) Nature. 388(6645): 839-840. 6. Mezey E., et al. (1998) Nat Med. 4(7): 755-757. |
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