Trypsin-EDTA (0.05%), phenol red胰蛋白酶
描述
Gibco® Trypsin-EDTA is made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength, trypsin is widely used for cell dissociation, routine cell culture passaging, and primary tissue dissociation. The trypsin concentration required for dissociation varies with cell type and experimental requirements. See the complete range of Gibco® trypsin solutions and recommended dissociation conditions.
We offer a variety of trypsin formulations and animal origin-free TrypLE™ reagent that feature:
Wu Yaojiong; Chen Liwen; Zheng Peng-Sheng; Yang Burton B;
杂志
J Biol Chem (2002) 277:12294-12595
产品用途
Lipofectin, Geneticin (G418), Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), Hanks' balanced salt solution, and trypsin/EDTA were from Invitrogen
Doi Junko; Takemori Hiroshi; Lin Xing-Zi; Horike Nanao; Katoh Yoshiko; Okamoto Mitsuhiro;
杂志
J Biol Chem (2002) 277:15629-15666
产品用途
To introduce DNAs into Y1 cells, LipofectAMINE 2000 was used in this study. Y1 cells were maintained in Dulbecco's modified Eagle's mediumtrypsin-EDTA from Invitrogen
Lee Vivian; Chen Liwen; Paiwand Frouz; Cao Liu; Wu Yaojiong; Inman Robert; Adams Mark E; Yang Burton B;
杂志
J Biol Chem (2002) 277:22279-22367
产品用途
Hanks' balanced salt solution, trypsin/EDTA, and T4 DNA ligase were from Invitrogen. To analyze gene expression, COS-7 cells or bovine chondrocytes were transfected transiently with recombinant constructs using Lipofectin according to the manufacturer's instructions (Invitrogen).