简单描述:This protein has been purified from mouse Ehrlich ascites cells by HPLC (see Kuznicki et al. (1989) Biochem. J. 263: 951-956). This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection.For fluid-phase adsorption in immunohistochemistry we suggest the following procedure:- Dilute 1 µl of the antiserum against S100A6 (calcyclin) (Swant) in 5 ml of the usual buffer forimmunohistochemistry (final dilution 1:5'000).- Add 1 µg of the purified protein to 1 ml of the diluted antibody solution and mix well.- Incubate for at least 6 hours in the cold.- Apply to tissue-sections and incubate for 3 days.- Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB).As a result, the immunostaining should be strongly reduced or even completely prevented.