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Recombinant human CD44
IgG2a
Lyophilized or Liquid
Store at -20 °C for one year.
单克隆
无
Human,
1年
bsm-51065M
999
北京博奥森生物技术有限公司
Mouse
WB=1:200-2000,IHC-P=1:400-800,IHC-F=1:20-200,IF=1:20-200,ICC=1:100-500,
1mg/ml
CD44
小鼠抗CD44单克隆抗体
 Sample: Lane 1: MDA-MB-231 (Human) Cell Lysate at 30 ug Lane 2: U251 (Human) Cell Lysate at 30 ug Lane 3: A549 (Human) Cell Lysate at 30 ug Primary: Anti-CD44 (bsm-51065M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 82 kD Observed band size: 85 kD  Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD44) Polyclonal Antibody, Unconjugated (bsm-51065M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD44) Polyclonal Antibody, Unconjugated (bsm-51065M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD44) Polyclonal Antibody, Unconjugated (bsm-51065M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (Human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD44) Monoclonal Antibody, Unconjugated (bsm-51065M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining. 已发表文献 [IF=1.851]Xie P et al. Therapeutic effect of transplantation of human bone marrow‑derived mesenchymal stem cells on neuron regeneration in a rat model of middle cerebral artery occlusion. Mol Med Rep. 2019 Jul 30. ICF, Human |
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