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发货周期:现货
博士德生物
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
96T
货号 | EK0370 |
---|---|
产品名称 | Human ICAM1 ELISA Kit |
产品描述 | For quantitative detection of human ICAM-1 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, Q28-E480 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 156pg/ml-10000pg/ml |
基因名称 | ICAM1 |
基因全名 | intercellular adhesion molecule 1 |
Uniprot ID | P05362 |
指标别名 | BB2|CD 54|CD54|CD54 antigen|Cell surface glycoprotein P3.58|Human rhinovirus receptor|ICAM 1|Intercellular adhesion molecule 1|Major group rhinovirus receptor|MALA2|MGC6195|MyD10|P3.58|Surface antigen of activated B cells |
背景资料 | Intercellular adhesion molecule-1(ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated(LFA) antigens, a beta 2 leukocyte integrin. The normal function of human intercellular adhesion molecule-1(ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen(LFA-1) or macrophage-1 antigen(Mac-1). ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines. ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class II antigens and is likely to play a role in inflammatory responses.1 |
显色时间 | 15-20分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0370-CAP | 96-well plate precoated with anti- human ICAM-1 antibody | 1 |
EK0370-ST | Lyophilized recombinant human ICAM-1 standard | 10ng/tube×2 |
EK0370-DA | Biotinylated anti- human ICAM-1 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 272 | 1923 | 4746 | 248 | 1929 | 4549 |
Standard deviation | 14.41 | 111.53 | 204.07 | 14.13 | 125.38 | 236.54 |
CV(%) | 5.3% | 5.8% | 4.3% | 5.7% | 6.5% | 5.2% |
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.006 | 0.088 | 0.187 | 0.408 | 0.742 | 1.351 | 1.884 | 2.392 |
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