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发货周期:现货
博士德生物
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
96T
货号 | EK0537 |
---|---|
产品名称 | Human VCAM-1 ELISA Kit |
产品描述 | For quantitative detection of human VCAM-1 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
免疫原 | NSO,F25 - E698 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 156pg/ml-10000pg/ml |
基因名称 | VCAM1 |
基因全名 | vascular cell adhesion molecule 1 |
Uniprot ID | P19320 |
指标别名 | CD106|CD106 antigen|DKFZp779G2333|INCAM 100|INCAM-100|INCAM100|L1CAM|MGC99561|V CAM 1|V-CAM 1|Vascular cell adhesion molecule 1|Vascular cell adhesion protein 1|VCAM 1|VCAM-1|VCAM1|VCAM1_HUMAN |
背景资料 | Vascular cell adhesion molecule 1(VCAM-1) is a cell surface glycoprotein adhesive for certain blood leukocytes and tumor cells, which is expressed by activated endothelium in a variety of pathologic conditions including atherosclerosis. Increased expression of VCAM1 is associated with a variety of chronic inflammatory conditions, making its expression and function a target for therapeutic intervention. Integrin alpha4beta1(VLA-4) and VCAM-1 facilitate a critical cell-cell adhesion event required for survival of endothelial and mural cells during vascularization. The VCAM1 gene is assigned to the 1p31-32 region of chromosome 1 based on the analysis of human-mouse hybrid cell lines and in situ hybridization. The standard product used in this kit is recombinant human VCAM-1, consisting of Phe25-Glu698 amino acid sequence of a single chain with the molecular mass of 74KDa. As a result of glycosylation, the molecular mass of 90-95KDa is revealed by SDS-PAGE. |
显色时间 | 25-30分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0537-CAP | 96-well plate precoated with anti- human VCAM-1 antibody | 1 |
EK0537-ST | Lyophilized recombinant human VCAM-1 standard | 10ng/tubex2 |
EK0537-DA | Biotinylated anti- human VCAM-1 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 310 | 1065 | 5287 | 304 | 1029 | 5603 |
Standard deviation | 17.67 | 51.12 | 385.95 | 19.15 | 65.85 | 481.85 |
CV(%) | 5.7% | 4.8% | 7.3% | 6.3% | 6.4% | 8.6% |
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.067 | 0.129 | 0.224 | 0.419 | 0.642 | 1.179 | 1.807 | 2.284 |
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