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发货周期:现货
博士德生物
cell culture supernates, cell lysates, serum.
Mouse
96T
货号 | EK0375 |
---|---|
产品名称 | Mouse IFN Gamma/IFNG ELISA Kit |
产品描述 | For quantitative detection of mouse IFN gamma in cell culture supernates and serum. |
免疫原 | E.coli, H23-C155 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <5pg/ml |
检测范围 | 31.2pg/ml-2000pg/ml |
基因名称 | IFNG |
基因全名 | interferon, gamma |
Uniprot ID | IFNG: P01580 |
指标别名 | IFG|IFI|IFN gamma|IFN Immune|IFNG|Interferon gamma|Interferon Gamma Precursor|Macrophage Activating Factor|MAF|T Cell Interferon|Type II Interferon |
背景资料 | Interferon-gamma(IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer(NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation. |
显色时间 | 25-30分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0375-CAP | 96-well plate precoated with anti- mouse IFN gamma antibody | 1 |
EK0375-ST | Lyophilized recombinant mouse IFN gamma standard | 10ng/tube×2 |
EK0375-DA | Biotinylated anti- mouse IFN gamma antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 68 | 224 | 760 | 70 | 232 | 686 |
Standard deviation | 2.92 | 12.32 | 33.44 | 3.08 | 12.76 | 30.18 |
CV(%) | 4.3% | 5.5% | 4.4% | 4.4% | 5.5% | 4.4% |
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.049 | 0.142 | 0.253 | 0.375 | 0.595 | 1.015 | 1.355 | 1.997 |
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