简单描述:Produced from sera of rabbits pre-immunized with highly pure recombinant Human FGF-acidic. Anti-Human FGF-acidic specific antibody was purified by affinity chromatography employing immobilized Human FGF-acidic matrix.
Immunohistochemistery: This antibody stained formalin-fixed, paraffin-embedded sections of human normal kidney. The recommended concentration is 0.050 µg/mL-0.125 µg/mL with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
Sandwich ELISA: To detect Human FGF-acidic by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human FGF-acidic (60-132BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human FGF-acidic.
Western Blot: To detect Human FGF-acidic by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human FGF-acidic is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human FGF-acidic (10.0 ng/ml), a concentration of 1.0-2.5 µg/ml of this antibody is required.