Chicken intestinal epithelial cells were obtained from NEWGAINBIO company. Cells were cultured on 37℃,with 5% CO2,in the Ham’s F-12 Nutrient(DMEM/F12)that contained the following supplementations:fetal bovine serum(5%),in-sulin(5 µg/mL ),transferring(5µg/mL),selenium(5ng/mL),epidermal growth factor(5ng/mL)and penicillin-streptomycin(100-100U/mL) for cell culturing(full DMEM/F12).