Reconstitute and make small portions upon arrival (e.g. 2-5 ul). For long storage, keep at - 80°C (or at least - 20°C). For continuous use, keep at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing.
克隆性:
多克隆
适应物种:
Goat
供应商:
安诺伦(北京)生物科技有限公司
靶点:
Parvalbumin
规格:
200 ul.
商品分类:Antibodies against Ca2+ -bindin
研究领域:Parvalbumin
存储溶液:Lyophilized antiserum (no preservatives).
参考文献:1.) Schwaller B., et al. (1999) Am. J. Physiol. 276. C395-403
应用:Immunohistochemistry : 1:2'000 - 1:5'000 with the avidin-biotin method. Immunoblots : 1:500 - 1:1'000 We recommend that the optimal dilutions be determined by titration experiments.
简单描述:This antiserum was produced against rat muscle parvalbumin. It cross-react with some other species (see immunoblot in Fig 1), including human parvalbumin. It can be used for immunoblotting and immunohistochemistry.Fig. 1 Immunoblot of brain homogenate of various species with the goat anti-parvalbuminantiserum1: Mouse; 2: Rat; 3. Guinea pig; 4. Rabbit; 5. Macaca fascicularis; 6. Zebrafish; 7.chicken. In the first five species, only a band at 12 KDa is detected. The second band inlanes 1 and 2 is a dimer of PV at approximately 24 KDa.
Lot No: 076
Reconstitution: with 200 ul bidistilled water.
Immunohistochemistry on Calbindin D-28k knock-out mice: Antiserum PVG 213 does not stain the brain of parvalbumin knock out mice (Figs. 2a and 2b).