简单描述:This recombinant protein has been purified by HPLC. This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection.For adsorption we suggest the following procedure:- Dilute 1 µl of the antibody 465 (Swant) against calmodulin in 5 ml of the usual buffer for immunohistochemistry(final dilution 1:5'000).- Add 1 µg of the recombinant protein to 1 ml of the diluted antibody solution and mix well.- Incubate for at least 6 hours in the cold.- Apply to tissue-sections and incubate for 3 days.- Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB).As a result, the immunostaining should be strongly reduced or even completely prevented.