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发货周期:现货
博士德生物
cell culture supernates, serum, plasma(EDTA) and urine.
96T
货号 | EK0513 |
---|---|
产品名称 | Human TGF beta 1 ELISA Kit |
产品描述 | For quantitative detection of activated human TGF beta 1 in cell culture supernates, serum, plasma(EDTA) and urine. |
免疫原 | CHO, A279-S390 |
交叉反应 | There is cross-reactivates with TGF beta 2, TGF beta 3, TGF beta 5<1%. |
敏感性 | <1pg/ml |
检测范围 | 15.6pg/ml-1000pg/ml |
基因名称 | TGFB1 |
基因全名 | transforming growth factor, beta 1 |
Uniprot ID | P01137 |
指标别名 | Camurati-Engelmann disease|CED|DPD1|LAP|Latency-associated peptide|TGF beta 1 protein|TGF-beta-1|TGFB|tgfb1|TGFB1_HUMAN|TGFbeta|Transforming growth factor beta 1 |
背景资料 | Transforming growth factor-beta1(TGF-beta1) is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. Many cells synthesize TGF-beta and essentially all of them have specific receptors for this peptide. TGF-beta regulates the actions of many other peptide growth factors and determines a positive or negative direction of their effects.TGFbeta1 is known for its potent and diverse biological effects, including immune regulation, and cell growth and differentiation.TGFbeta1 is also an important mediator of bone remodeling.TGFbeta1, a potent keratinocyte growth inhibitor, has been shown to be overexpressed in keratinocytes in certain inflammatory skin diseases and has been thought to counteract the effects of other growth factors at the site of inflammation.TGF-beta1, a multifunctional cytokine with fibrogenic properties, has been implicated in the pathogenesis of the vascular and target organ complications of hypertension. TGF-beta1 may also regulate blood pressure via stimulation of endothelin-1 and/or renin secretion. TGFbeta1 is secreted as a latent form, which consists of its mature form and a latency-associated peptide(beta1-LAP) in either the presence or the absence of additional latent TGF-beta1-binding protein. The standard product used in this kit is recombinant TGFbeta1 with the molecular mass of 25KDa. |
显色时间 | 15-20分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0513-CAP | 96-well plate precoated with anti- human TGF beta 1 antibody | 1 |
EK0513-ST | Lyophilized recombinant human TGF beta 1 standard | 10ng/tubex2 |
EK0513-DA | Biotinylated anti- human TGF beta 1 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 30 | 156 | 497 | 28 | 164 | 537 |
Standard deviation | 1.38 | 8.42 | 34.79 | 1.73 | 8.85 | 38.12 |
CV(%) | 4.6% | 5.4% | 7% | 6.2% | 5.4% | 7.1% |
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.039 | 0.108 | 0.186 | 0.321 | 0.599 | 1.017 | 1.586 | 2.200 |
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