Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep,
产品应用
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分 子 量
54kDa
细胞定位
细胞浆 细胞膜
性 状
Lyophilized or Liquid
浓 度
1mg/ml
免 疫 原
KLH conjugated synthetic peptide derived from human GLRB:51-150/497 <Extracellular>
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed
PubMed
产品介绍
background: GLRB (Glycine receptor beta) is a neurotransmitter-gated ion channel concentrated within the spinal cord and brainstem. Expression is also observed in several upper brain regions including the cortex, cerebellum, hippocampus and amygdala. Binding of glycine to GLRB increases the chloride conductance and thus produces hyperpolarization (inhibition of neuronal firing), controlling spinal reflexes and locomotor behavior.
Function: The glycine receptor is a neurotransmitter-gated ion channel. Binding of glycine to its receptor increases the chloride conductance and thus produces hyperpolarization (inhibition of neuronal firing).
Subunit: Pentamer composed of alpha and beta subunits. Interacts with GPHN
DISEASE: Defects in GLRB are the cause of hyperekplexia type 2 (HKPX2) [MIM:614619]. HKPX2 is a neurologic disorder characterized by muscular rigidity of central nervous system origin, particularly in the neonatal period, and by an exaggerated startle response to unexpected acoustic or tactile2 stimuli.
Similarity: Belongs to the ligand-gated ion channel (TC 1.A.9) family. Glycine receptor (TC 1.A.9.3) subfamily. GLRB sub-subfamily.
SWISS: P48167
Gene ID: 2743
Database links:
Entrez Gene: 2743 Human
Entrez Gene: 14658 Mouse
Entrez Gene: 25456 Rat
Omim: 138492 Human
SwissProt: P48167 Human
SwissProt: P48168 Mouse
SwissProt: P20781 Rat
Unigene: 32973 Human
Unigene: 275639 Mouse
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品图片
Sample: Cerebrum (Mouse)Lysate at 40 ug Cerebellum (Mouse)Lysate at 40 ug Primary: Anti-GLRB(bs-20450R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 54kD Observed band size: 54kD