IHC-P=1:400-800 IHC-F=1:400-800 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分 子 量
53kDa
细胞定位
细胞浆
性 状
Lyophilized or Liquid
浓 度
1mg/ml
免 疫 原
KLH conjugated synthetic peptide derived from human Epsilon Tubulin:
亚 型
IgG
纯化方法
affinity purified by Protein G
储 存 液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed
PubMed
产品介绍
background: This gene encodes a member of the tubulin superfamily. This protein localizes to the centriolar sub-distal appendages that are associated with the older of the two centrioles after centrosome duplication. This protein plays a central role in organization of the microtubules during centriole duplication. A pseudogene of this gene is found on chromosome 5.[provided by RefSeq, Jan 2009]
Subcellular Location: Cytoplasm; cytoskeleton; centrosome. Associated with pericentriolar material.
Similarity: Belongs to the tubulin family.
SWISS: Q9UJT0
Gene ID: 51175
Database links:
Entrez Gene: 51175 Human
Omim: 607345 Human
SwissProt: Q9UJT0 Human
Unigene: 34851 Human
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品图片
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TBE) Polyclonal Antibody, Unconjugated (bsm-33314M-4C3) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.