大家都在搜
手机验证
发货周期:现货
博士德生物
cell culture supernates, serum and plasma(heparin, EDTA, citrate).
Mouse
96T
货号 | EK0470 |
---|---|
产品名称 | Mouse NGF/NGF beta ELISA Kit |
产品描述 | For quantitative detection of mouse NGF in cell culture supernates, serum and plasma(heparin, EDTA, citrate). |
免疫原 | NSO, S122-G241 |
交叉反应 | There is no detectable cross-reactivity with BDGF, GDNF, CNTF, NT3 and NT4. |
敏感性 | <1pg/ml |
检测范围 | 31.2pg/ml-2000pg/ml |
基因名称 | NGF |
基因全名 | nerve growth factor (beta polypeptide) |
Uniprot ID | P01139 |
指标别名 | Beta nerve growth factor|Beta NGF|HSAN5|MGC161426|MGC161428|Nerve growth factor (beta polypeptide)|Nerve growth factor beta polypeptide|Nerve growth factor beta subunit|NGFB|NID67 |
背景资料 | Nerve growth factor(NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male mouse submaxillary gland(MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight(Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF. NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis. |
显色时间 | 20-25分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0470-CAP | 96-well plate precoated with anti- mouse NGF antibody | 1 |
EK0470-ST | Lyophilized recombinant mouse NGF standard | 10ng/tubex2 |
EK0470-DA | Biotinylated anti- mouse NGF antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 99 | 235 | 910 | 99 | 244 | 967 |
Standard deviation | 6.43 | 17.39 | 70.07 | 6.43 | 18.05 | 80.26 |
CV(%) | 6.5% | 7.4% | 7.7% | 6.5% | 7.4% | 8.3% |
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.033 | 0.110 | 0.180 | 0.377 | 0.576 | 1.056 | 1.727 | 2.454 |
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。